MFN1 antibody (AA 1-741)

Catalog No. ABIN527615

Product Details anti-MFN1 Antibody

Target: MFN1 (Mitofusin 1 (MFN1))

Binding Specificity: AA 1-741

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MFN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, RNA Interference (RNAi)

Purpose: Mouse monoclonal antibody raised against a full length recombinant MFN1.

Sequence: MAEPVSPLKH FVLAKKAITA IFDQLLEFVT EGSHFVEATY KNPELDRIAT EDDLVEMQGY KDKLSIIGEV LSRRHMKVAF FGRTSSGKSS VINAMLWDKV LPSGIGHITN CFLSVEGTDG DKAYLMTEGS DEKKSVKTVN QLAHALHMDK DLKAGCLVRV FWPKAKCALL RDDLVLVDSP GTDVTTELDS WIDKFCLDAD VFVLVANSES TLMNTEKHFF HKVNERLSKP NIFILNNRWD ASASEPEYME DVRRQHMERC LHFLVEELKV VNALEAQNRI FFVSAKEVLS ARKQKAQGMP ESGVALAEGF HARLQEFQNF EQIFEECISQ SAVKTKFEQH TIRAKQILAT VKNIMDSVNL AAEDKRHYSV EEREDQIDRL DFIRNQMNLL TLDVKKKIKE VTEEVANKVS CAMTDEICRL SVLVDEFCSE FHPNPDVLKI YKSELNKHIE DGMGRNLADR CTDEVNALVL QTQQEIIENL KPLLPAGIQD KLHTLIPCKK FDLSYNLNYH KLCSDFQEDI VFRFSLGWSS LVHRFLGPRN AQRVLLGLSE PIFQLPRSLA STPTAPTTPA TPDNASQEEL MITLVTGLAS VTSRTSMGII IVGGVIWKTI GWKLLSVSLT MYGALYLYER LSWTTHAKER AFKQQFVNYA TEKLRMIVSS TSANCSHQVK QQIATTFARL CQQVDITQKQ LEEEIARLPK EIDQLEKIQN NSKLLRNKAV QLENELENFT KQFLPSSNEE S

Specificity: This antibody may cross react with MFN2 protein.

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: MFN1 (AAH40557, 1 a.a. ~ 741 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 3C9

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-MFN1 antibody (ABIN527615)

Chen, Chen, Wang, Tan, Zhu, Li, Han, Chen, Gao, Liu, Chen: "Mitophagy receptor FUNDC1 regulates mitochondrial dynamics and mitophagy." in: Autophagy, Vol. 12, Issue 4, pp. 689-702, (2016) (PubMed).

Baba, Kashiwagi, Arimitsu, Kogure, Edo, Maruyama, Nakao, Nakanishi, Kinoshita, Frohman, Yamamoto, Tani: "Phosphatidic acid (PA)-preferring phospholipase A1 regulates mitochondrial dynamics." in: The Journal of biological chemistry, Vol. 289, Issue 16, pp. 11497-511, (2014) (PubMed).

Yue, Chen, Liu, Yan, Chen, Feng, Yan, Wu, Du, Wang, Liu, Huang, Xia, Liu, Wang, Jin, Wang, Song, Hao, Chen: "A small natural molecule promotes mitochondrial fusion through inhibition of the deubiquitinase USP30." in: Cell research, Vol. 24, Issue 4, pp. 482-96, (2014) (PubMed).

Shiba-Fukushima, Inoshita, Hattori, Imai: "Lysine 63-linked polyubiquitination is dispensable for Parkin-mediated mitophagy." in: The Journal of biological chemistry, Vol. 289, Issue 48, pp. 33131-6, (2014) (PubMed).

Takamura, Koyama, Matsuzaki, Yamada, Hattori, Miyata, Takemoto, Tohyama, Katayama: "TRAP1 controls mitochondrial fusion/fission balance through Drp1 and Mff expression." in: PLoS ONE, Vol. 7, Issue 12, pp. e51912, (2013) (PubMed).

Goller, Seibold, Kremmer, Voos, Kolanus: "Atad3 function is essential for early post-implantation development in the mouse." in: PLoS ONE, Vol. 8, Issue 1, pp. e54799, (2013) (PubMed).

Russell, Lamon, Boon, Wada, Güller, Brown, Chibalin, Zierath, Snow, Stepto, Wadley, Akimoto: "Regulation of miRNAs in human skeletal muscle following acute endurance exercise and short-term endurance training." in: The Journal of physiology, Vol. 591, Issue Pt 18, pp. 4637-53, (2013) (PubMed).

Ban-Ishihara, Ishihara, Sasaki, Mihara, Ishihara: "Dynamics of nucleoid structure regulated by mitochondrial fission contributes to cristae reformation and release of cytochrome c." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 29, pp. 11863-8, (2013) (PubMed).

Fülöp, Rajki, Katona, Szanda, Spät: "Extramitochondrial OPA1 and adrenocortical function." in: Molecular and cellular endocrinology, Vol. 381, Issue 1-2, pp. 70-9, (2013) (PubMed).

Lin, Lai, Lin, Lin, Wang, Lin, Lee, Wang, Chen: "Suppressor of cytokine signaling 6 (SOCS6) promotes mitochondrial fission via regulating DRP1 translocation." in: Cell death and differentiation, Vol. 20, Issue 1, pp. 139-53, (2012) (PubMed).

Shiba-Fukushima, Imai, Yoshida, Ishihama, Kanao, Sato, Hattori: "PINK1-mediated phosphorylation of the Parkin ubiquitin-like domain primes mitochondrial translocation of Parkin and regulates mitophagy." in: Scientific reports, Vol. 2, pp. 1002, (2012) (PubMed).

Bui, Gilady, Fitzsimmons, Benson, Lynes, Gesson, Alto, Strack, Scott, Simmen: "Rab32 modulates apoptosis onset and mitochondria-associated membrane (MAM) properties." in: The Journal of biological chemistry, Vol. 285, Issue 41, pp. 31590-602, (2010) (PubMed).

Zanna, Ghelli, Porcelli, Karbowski, Youle, Schimpf, Wissinger, Pinti, Cossarizza, Vidoni, Valentino, Rugolo, Carelli: "OPA1 mutations associated with dominant optic atrophy impair oxidative phosphorylation and mitochondrial fusion." in: Brain : a journal of neurology, Vol. 131, Issue Pt 2, pp. 352-67, (2008) (PubMed).

Holloway, Perry, Thrush, Heigenhauser, Dyck, Bonen, Spriet: "PGC-1alpha's relationship with skeletal muscle palmitate oxidation is not present with obesity despite maintained PGC-1alpha and PGC-1beta protein." in: American journal of physiology. Endocrinology and metabolism, Vol. 294, Issue 6, pp. E1060-9, (2008) (PubMed).

Target Details for MFN1

Target: MFN1 (Mitofusin 1 (MFN1))

Alternative Name: MFN1 (MFN1 Products)

Synonyms: mfn1 antibody, zgc:66150 antibody, mitofusin-1 antibody, MFN1 antibody, MFN2 antibody, hfzo1 antibody, hfzo2 antibody, 2310002F04Rik antibody, 6330416C07Rik antibody, D3Ertd265e antibody, HR2 antibody, mKIAA4032 antibody, Fzo1b antibody, mitofusin 1b antibody, mitofusin 1 antibody, mitofusin 1 S homeolog antibody, mfn1b antibody, MFN1 antibody, mfn1.S antibody, mfn1 antibody, Mfn1 antibody

Background: Full Gene Name: mitofusin 1
Synonyms: DKFZp762F247,FLJ20693,MGC41806,hfzo1,hfzo2

Gene ID: 55669