RNF111 antibody (AA 1-108)

Catalog No. ABIN527194

Product Details anti-RNF111 Antibody

Target: RNF111 (Ring Finger Protein 111 (RNF111))

Binding Specificity: AA 1-108

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RNF111 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), RNA Interference (RNAi)

Purpose: Mouse monoclonal antibody raised against a partial recombinant RNF111.

Sequence: MSQWTPEYNE LYTLKVDMKS EIPSDAPKTQ ESLKGILLHP EPIGAAKSFP AGVEMINSKV GNEFSHLCDD SQKQEKEMNG NQQEQEKSLV VRKKRKSQQA GPSYVQNC

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: RNF111 (NP_060080, 1 a.a. ~ 108 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 1C4

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-RNF111 antibody (ABIN527194)

van Cuijk, van Belle, Turkyilmaz, Poulsen, Janssens, Theil, Sabatella, Lans, Mailand, Houtsmuller, Vermeulen, Marteijn: "SUMO and ubiquitin-dependent XPC exchange drives nucleotide excision repair." in: Nature communications, Vol. 6, pp. 7499, (2015) (PubMed).

Ma, Chen, Zhang, Yang, Wang, Yu: "RNF111-dependent neddylation activates DNA damage-induced ubiquitination." in: Molecular cell, Vol. 49, Issue 5, pp. 897-907, (2013) (PubMed).

Erker, Neyret-Kahn, Seeler, Dejean, Atfi, Levy: "Arkadia, a novel SUMO-targeted ubiquitin ligase involved in PML degradation." in: Molecular and cellular biology, Vol. 33, Issue 11, pp. 2163-77, (2013) (PubMed).

Poulsen, Hansen, Wagner, van Cuijk, van Belle, Streicher, Wikström, Choudhary, Houtsmuller, Marteijn, Bekker-Jensen, Mailand: "RNF111/Arkadia is a SUMO-targeted ubiquitin ligase that facilitates the DNA damage response." in: The Journal of cell biology, Vol. 201, Issue 6, pp. 797-807, (2013) (PubMed).

Javelaud, van Kempen, Alexaki, Le Scolan, Luo, Mauviel: "Efficient TGF-?/SMAD signaling in human melanoma cells associated with high c-SKI/SnoN expression." in: Molecular cancer, Vol. 10, Issue 1, pp. 2, (2011) (PubMed).

Koinuma, Shinozaki, Nagano, Ikushima, Horiguchi, Goto, Chano, Saitoh, Imamura, Miyazono, Miyazawa: "RB1CC1 protein positively regulates transforming growth factor-beta signaling through the modulation of Arkadia E3 ubiquitin ligase activity." in: The Journal of biological chemistry, Vol. 286, Issue 37, pp. 32502-12, (2011) (PubMed).

Nagano, Koinuma, Miyazawa, Miyazono: "Context-dependent regulation of the expression of c-Ski protein by Arkadia in human cancer cells." in: Journal of biochemistry, Vol. 147, Issue 4, pp. 545-54, (2010) (PubMed).

Nanjundan, Cheng, Zhang, Lahad, Kuo, Schmandt, Smith-McCune, Fishman, Gray, Mills: "Overexpression of SnoN/SkiL, amplified at the 3q26.2 locus, in ovarian cancers: a role in ovarian pathogenesis." in: Molecular oncology, Vol. 2, Issue 2, pp. 164-81, (2009) (PubMed).

Levy, Howell, Das, Harkin, Episkopou, Hill: "Arkadia activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation." in: Molecular and cellular biology, Vol. 27, Issue 17, pp. 6068-83, (2007) (PubMed).

Target Details for RNF111

Target: RNF111 (Ring Finger Protein 111 (RNF111))

Alternative Name: RNF111 (RNF111 Products)

Background: Full Gene Name: ring finger protein 111
Synonyms: ARK,DKFZp313E0731,DKFZp686H1966,DKFZp761D081,FLJ38008

Gene ID: 54778

NCBI Accession: NM_017610