B3GAT3 antibody (AA 1-335)

Catalog No. ABIN525639

Product Details anti-B3GAT3 Antibody

Target: B3GAT3 (beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3))

Binding Specificity: AA 1-335

Reactivity: Human

Host: Mouse

Clonality: Polyclonal

Conjugate: This B3GAT3 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Mouse polyclonal antibody raised against a full-length human B3GAT3 protein.

Sequence: MKLKLKNVFL AYFLVSIAGL LYALVQLGQP CDCLPPLRAA AEQLRQKDLR ISQLQAELRR PPPAPAQPPE PEALPTIYVV TPTYARLVQK AELVRLSQTL SLVPRLHWLL VEDAEGPTPL VSGLLAASGL LFTHLVVLTP KAQRLREGEP GWVHPRGVEQ RNKALDWLRG RGGAVGGEKD PPPPGTQGVV YFADDDNTYS RELFEEMRWT RGVSVWPVGL VGGLRFEGPQ VQDGRVVGFH TAWEPSRPFP VDMAGFAVAL PLLLDKPNAQ FDSTAPRGHL ESSLLSHLVD PKDLEPRAAN CTRVLVWHTR TEKPKMKQEE QLQRQGRGSD PAIEV

Cross-Reactivity: Human

Characteristics: Antibody reactive against mammalian transfected lysate.

Immunogen: B3GAT3 (NP_036332.2, 1 a.a. ~ 335 a.a) full-length human protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-B3GAT3 antibody (ABIN525639)

Job, Mizumoto, Smith, Couser, Brazil, Saal, Patterson, Gibson, Soden, Miller, Thiffault, Saunders, Yamada, Hoffmann, Sugahara, Farrow: "Functional validation of novel compound heterozygous variants in B3GAT3 resulting in severe osteopenia and fractures: expanding the disease phenotype." in: BMC medical genetics, Vol. 17, Issue 1, pp. 86, (2016) (PubMed).

Budde, Mizumoto, Kogawa, Becker, Altmüller, Thiele, Rüschendorf, Toliat, Kaleschke, Hämmerle, Höhne, Sugahara, Nürnberg, Kennerknecht: "Skeletal dysplasia in a consanguineous clan from the island of Nias/Indonesia is caused by a novel mutation in B3GAT3." in: Human genetics, Vol. 134, Issue 7, pp. 691-704, (2015) (PubMed).

Baasanjav, Al-Gazali, Hashiguchi, Mizumoto, Fischer, Horn, Seelow, Ali, Aziz, Langer, Saleh, Becker, Nürnberg, Cantagrel, Gleeson, Gomez, Michel, Stricker, Lindner, Nürnberg, Sugahara, Mundlos et al.: "Faulty initiation of proteoglycan synthesis causes cardiac and joint defects. ..." in: American journal of human genetics, Vol. 89, Issue 1, pp. 15-27, (2011) (PubMed).

Hiyama, Gajghate, Sakai, Mochida, Shapiro, Risbud: "Activation of TonEBP by calcium controls {beta}1,3-glucuronosyltransferase-I expression, a key regulator of glycosaminoglycan synthesis in cells of the intervertebral disc." in: The Journal of biological chemistry, Vol. 284, Issue 15, pp. 9824-34, (2009) (PubMed).

Target Details for B3GAT3

Target: B3GAT3 (beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3))

Alternative Name: B3GAT3 (B3GAT3 Products)

Background: Full Gene Name: beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I)
Synonyms: GLCATI,GlcAT-I

Gene ID: 26229

NCBI Accession: NM_012200

Pathways: Glycosaminoglycan Metabolic Process