TFAM antibody (AA 1-246)

Catalog No. ABIN520832

Product Details anti-TFAM Antibody

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Binding Specificity: AA 1-246

Reactivity: Human

Host: Mouse

Clonality: Polyclonal

Conjugate: This TFAM antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Mouse polyclonal antibody raised against a full-length human TFAM protein.

Sequence: MAFLRSMWGV LSALGRSGAE LCTGCGSRLR SPFSFVYLPR WFSSVLASCP KKPVSSYLRF SKEQLPIFKA QNPDAKTTEL IRRIAQRWRE LPDSKKKIYQ DAYRAEWQVY KEEISRFKEQ LTPSQIMSLE KEIMDKHLKR KAMTKKKELT LLGKPKRPRS AYNVYVAERF QEAKGDSPQE KLKTVKENWK NLSDSEKELY IQHAKEDETR YHNEMKSWEE QMIEVGRKDL LRRTIKKQRK YGAEEC

Cross-Reactivity: Human

Characteristics: Antibody reactive against mammalian transfected lysate.

Immunogen: TFAM (NP_003192.1, 1 a.a. ~ 246 a.a) full-length human protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-TFAM antibody (ABIN520832)

Takeda, Hasegawa, Ueha, Sakakibara, Kawamoto, Minamikawa, Sakai, Komori: "Decreased mitochondrial copy numbers in oral squamous cell carcinoma." in: Head & neck, Vol. 38, Issue 8, pp. 1170-5, (2016) (PubMed).

Morishita, Kawamoto, Hara, Onishi, Ueha, Minoda, Katayama, Takemori, Fukase, Kurosaka, Kuroda, Akisue: "AICAR induces mitochondrial apoptosis in human osteosarcoma cells through an AMPK-dependent pathway." in: International journal of oncology, Vol. 50, Issue 1, pp. 23-30, (2016) (PubMed).

Akbari, Sykora, Bohr: "Slow mitochondrial repair of 5'-AMP renders mtDNA susceptible to damage in APTX deficient cells." in: Scientific reports, Vol. 5, pp. 12876, (2015) (PubMed).

Akbari, Keijzers, Maynard, Scheibye-Knudsen, Desler, Hickson, Bohr: "Overexpression of DNA ligase III in mitochondria protects cells against oxidative stress and improves mitochondrial DNA base excision repair." in: DNA repair, Vol. 16, pp. 44-53, (2014) (PubMed).

Gibellini, Pinti, Boraldi, Giorgio, Bernardi, Bartolomeo, Nasi, De Biasi, Missiroli, Carnevale, Losi, Tesei, Pinton, Quaglino, Cossarizza: "Silencing of mitochondrial Lon protease deeply impairs mitochondrial proteome and function in colon cancer cells." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 12, pp. 5122-35, (2014) (PubMed).

Gispert, Parganlija, Klinkenberg, Dröse, Wittig, Mittelbronn, Grzmil, Koob, Hamann, Walter, Büchel, Adler, Hrabé de Angelis, Busch, Zell, Reichert, Brandt, Osiewacz, Jendrach, Auburger: "Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors." in: Human molecular genetics, Vol. 22, Issue 24, pp. 4871-87, (2013) (PubMed).

Rolland, Motori, Memar, Hench, Frank, Winklhofer, Conradt: "Impaired complex IV activity in response to loss of LRPPRC function can be compensated by mitochondrial hyperfusion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 32, pp. E2967-76, (2013) (PubMed).

Holmström, Iglesias-Gutierrez, Zierath, Garcia-Roves: "Tissue-specific control of mitochondrial respiration in obesity-related insulin resistance and diabetes." in: American journal of physiology. Endocrinology and metabolism, Vol. 302, Issue 6, pp. E731-9, (2012) (PubMed).

Aamann, Sorensen, Hvitby, Berquist, Muftuoglu, Tian, de Souza-Pinto, Scheibye-Knudsen, Wilson, Stevnsner, Bohr: "Cockayne syndrome group B protein promotes mitochondrial DNA stability by supporting the DNA repair association with the mitochondrial membrane." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 7, pp. 2334-46, (2010) (PubMed).

Target Details for TFAM

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Alternative Name: TFAM (TFAM Products)

Synonyms: tcf6 antibody, mttf1 antibody, mttfa antibody, tcf6l1 antibody, tcf6l2 antibody, tcf6l3 antibody, mttfa-A antibody, xl-mtTFA antibody, MGC153358 antibody, zgc:153358 antibody, MTTF1 antibody, MTTFA antibody, TCF6 antibody, TCF6L1 antibody, TCF6L2 antibody, TCF6L3 antibody, AI661103 antibody, Hmgts antibody, mtTFA antibody, tsHMG antibody, Mttfa antibody, transcription factor A, mitochondrial antibody, transcription factor A, mitochondrial L homeolog antibody, TFAM antibody, tfam.L antibody, tfam antibody, Tfam antibody

Background: Full Gene Name: transcription factor A, mitochondrial
Synonyms: MtTF1,TCF6,TCF6L1,TCF6L2,TCF6L3,mtTFA

Gene ID: 7019

NCBI Accession: NM_003201

Pathways: Chromatin Binding