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HSP27 antibody (AA 96-205)

Name: HSP27 antibody (AA 96-205)
SKU: ABIN516740
Price: 651.43 USD
Availability: InStock

HSPB1 Reactivity: Human WB, ELISA, IF, IP, PLA Host: Mouse Monoclonal 3G3 unconjugated

Western Blot analysis of HSPB1 expression in transfected 293T cell line by HSPB1 monoclonal antibody (M04), clone 3G3. (HSP27 antibody (AA 96-205))

Detection limit for recombinant GST tagged HSPB1 is approximately 0. (HSP27 antibody (AA 96-205))

ELISA

Proximity Ligation Analysis of protein-protein interactions between AKT1 and HSPB1. (HSP27 antibody (AA 96-205))

PLA

Immunofluorescence of monoclonal antibody to HSPB1 on HeLa cell. (HSP27 antibody (AA 96-205))

Immunoprecipitation of HSPB1 transfected lysate using anti-HSPB1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with HSPB1 MaxPab rabbit polyclonal antibody. (HSP27 antibody (AA 96-205))

Western Blot detection against Immunogen (37. (HSP27 antibody (AA 96-205))

6 images

Catalog No. ABIN516740

Datasheet as PDF

Target See all HSP27 (HSPB1) Antibodies

HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
Binding Specificity
41

34

34

16

16

10

8

8

8

5

4

4

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 96-205
Reactivity
312

109

94

25

14

14

13

13

12

6

5

4

3

2

2

2

1
Human
Host
250

67
Mouse
Compatible Secondaries
Clonality
227

89
Monoclonal
Conjugate
137

23

16

13

11

7

6

6

6

6

6

6

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

2

1

1

1

1

1

1

1

1
This HSP27 antibody is un-conjugated
Application
255

111

98

93

63

50

48

44

41

40

15

12

10

7

7

3

2

2

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Proximity Ligation Assay (PLA)

Purpose

Mouse monoclonal antibody raised against a partial recombinant HSPB1.

Sequence

RVSLDVNHFA PDELTVKTKD GVVEITGKHE ERQDEHGYIS RCFTRKYTLP PGVDPTQVSS SLSPEGTLTV EAPMPKLATQ SNEITIPVTF ESRAQLGGPE AAKSDETAAK

Cross-Reactivity

Human

Characteristics

Antibody Reactive Against Recombinant Protein.

Immunogen

HSPB1 (NP_001531, 96 a.a. ~ 205 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone

3G3

Isotype

IgG2a

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anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 101-205) antibody
HSPB1 Reactivity: Human, Rat, Mouse WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

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HSPB1 Reactivity: Human, Rat WB, IHC, ELISA, FACS, ICC Host: Mouse Monoclonal 5D7 unconjugated

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HSPB1 Reactivity: Human WB, IHC, ELISA, IF, FACS, IP Host: Rabbit Monoclonal 10C11 unconjugated Recombinant Antibody

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HSPB1 Reactivity: Human WB, IHC, IF, FACS, IP, ICC Host: Mouse Monoclonal 8A7 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 Reactivity: Human, Rat, Mouse, Sheep, Monkey, Chicken, Chimpanzee WB, IHC, IF, FACS, ISt, StM Host: Mouse Monoclonal G3-1 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibody
HSPB1 Reactivity: Human, Rat, Mouse WB, IF, FACS, IHC (p), IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 Reactivity: Human WB, IHC, IF, FACS, ICC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 Reactivity: Human WB, IHC, ELISA, IF, IP, ICC Host: Mouse Monoclonal 5D12-A12 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (HRP)
HSPB1 Reactivity: Human WB, IHC, IF, FACS, IP, ICC Host: Mouse Monoclonal 8A7 HRP

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (FITC)
HSPB1 Reactivity: Human WB, IHC, IF, FACS, IP, ICC Host: Mouse Monoclonal 8A7 FITC

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Buffer

In 1x PBS, pH 7.4

Handling Advice

Aliquot to avoid repeated freezing and thawing.

Storage

-20 °C

Storage Comment

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Target

HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name

HSPB1 (HSPB1 Products)

Synonyms

CMT2F antibody, HMN2B antibody, HS.76067 antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, 27kDa antibody, Hsp27 antibody, cb153 antibody, cb660 antibody, hsp1 antibody, hsp25 antibody, hsp27 antibody, id:ibd2821 antibody, sb:cb660 antibody, zgc:103437 antibody, HSPB1 antibody, cmt2f antibody, hs.76067 antibody, hsp28 antibody, heat shock protein family B (small) member 1 antibody, heat shock protein 1 antibody, heat shock protein, alpha-crystallin-related, 1 antibody, heat shock protein family B (small) member 1 L homeolog antibody, HSPB1 antibody, Hspb1 antibody, hspb1 antibody, hspb1.L antibody

Background

Full Gene Name: heat shock 27 kDa protein 1
Synonyms: CMT2F,DKFZp586P1322,HMN2B,HS.76067,HSP27,HSP28,Hsp25,SRP27

Gene ID

3315

NCBI Accession

NM_001540

Pathways

MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling