CrkL antibody (AA 204-303)

Catalog No. ABIN514606

Product Details anti-CrkL Antibody

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Binding Specificity: AA 204-303

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CrkL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Proximity Ligation Assay (PLA), RNA Interference (RNAi)

Purpose: Mouse monoclonal antibody raised against a partial recombinant CRKL.

Sequence: AHAYAQPQTT TPLPAVSGSP GAAITPLPST QNGPVFAKAI QKRVPCAYDK TALALEVGDI VKVTRMNING QWEGEVNGRK GLFPFTHVKI FDPQNPDENE

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: CRKL (NP_005198, 204 a.a. ~ 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 4B5

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-CrkL antibody (ABIN514606)

Liu, Chen, Chau, Jan, Chen, Hsu, Lin, Juang, Lu, Cheng, Chen, Chang, Ting, Kao, Hsiao, Huang: "Analysis of protein-protein interactions in cross-talk pathways reveals CRKL protein as a novel prognostic marker in hepatocellular carcinoma." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 5, pp. 1335-49, (2013) (PubMed).

Segovis, Schoon, Dick, Nacusi, Leibson, Billadeau: "PI3K links NKG2D signaling to a CrkL pathway involved in natural killer cell adhesion, polarity, and granule secretion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 11, pp. 6933-42, (2009) (PubMed).

Target Details for CrkL

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Alternative Name: CRKL (CRKL Products)

Synonyms: CRKL antibody, wu:fa55g05 antibody, wu:fj60e01 antibody, zgc:63469 antibody, 1110025F07Rik antibody, AA589403 antibody, AI325100 antibody, Crkol antibody, CRK like proto-oncogene, adaptor protein antibody, v-crk avian sarcoma virus CT10 oncogene homolog-like antibody, CRKL antibody, crkl antibody, Crkl antibody

Background: Full Gene Name: v-crk sarcoma virus CT10 oncogene homolog (avian)-like
Synonyms:

Gene ID: 1399

NCBI Accession: NM_005207

Pathways: Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor