Cofilin antibody (AA 1-166)

Catalog No. ABIN514354

Product Details anti-Cofilin Antibody

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Binding Specificity: AA 1-166

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cofilin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Mouse monoclonal antibody raised against a full length recombinant CFL1.

Sequence: MASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE EGKEILVGDV GQTVDDPYAT FAKMLPDKDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKLTG IKHELQANCY EGVKDRCTLA EKLGGSAVIS LEGKPL

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: CFL1 (AAH11005, 1 a.a. ~ 166 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 1A1

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-Cofilin antibody (ABIN514354)

Chang, Chao, Lin, Tsai, Chou, Lee: "Determination of urine cofilin-1 level in acute kidney injury using a high-throughput localized surface plasmon-coupled fluorescence biosensor." in: Journal of biomedical optics, Vol. 19, Issue 1, pp. 011004, (2013) (PubMed).

Franciosi, Govorukhina, Fusetti, Poolman, Lodewijk, Timens, Postma, ten Hacken, Bischoff: "Proteomic analysis of human epithelial lining fluid by microfluidics-based nanoLC-MS/MS: a feasibility study." in: Electrophoresis, Vol. 34, Issue 18, pp. 2683-94, (2013) (PubMed).

Nishimura, Tsuda, Kataoka, Arao, Nomura, Chiyoda, Susumu, Nishio, Aoki: "Overexpression of cofilin 1 can predict progression-free survival in patients with epithelial ovarian cancer receiving standard therapy." in: Human pathology, Vol. 42, Issue 4, pp. 516-21, (2011) (PubMed).

Sakai, Otani, Miyamoto, Yoshida, Furuya, Tanigawa: "Identification of phosphorylated serine-15 and -82 residues of HSPB1 in 5-fluorouracil-resistant colorectal cancer cells by proteomics." in: Journal of proteomics, Vol. 75, Issue 3, pp. 806-18, (2011) (PubMed).

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: CFL1 (CFL1 Products)

Background: Full Gene Name: cofilin 1 (non-muscle)
Synonyms: CFL

Gene ID: 1072

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events