RUNX2 antibody (AA 251-350)

Catalog No. ABIN514045

Product Details anti-RUNX2 Antibody

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Binding Specificity: AA 251-350

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RUNX2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Mouse monoclonal antibody raised against a partial recombinant RUNX2.

Sequence: NPRPSLNSAP SPFNPQGQSQ ITDPRQAQSS PPWSYDQSYP SYLSQMTSPS IHSTTPLSST RGTGLPAITD VPRRISDDDT ATSDFCLWPS TLSKKSQAGA

Cross-Reactivity: Human, Rat

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: RUNX2 (NP_004339, 251 a.a. ~ 350 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 3F5

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-RUNX2 antibody (ABIN514045)

Sonomoto, Yamaoka, Kaneko, Yamagata, Sakata, Zhang, Kondo, Zenke, Sabanai, Nakayamada, Sakai, Tanaka: "Spontaneous Differentiation of Human Mesenchymal Stem Cells on Poly-Lactic-Co-Glycolic Acid Nano-Fiber Scaffold." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153231, (2016) (PubMed).

Li, Zhou, Takai, Sasaki, Mezawa, Li, Wang, Yang, Wang, Matsumura, Kaneko, Yoshimura, Ogata: "Aggregatibacter actinomycetemcomitans lipopolysaccharide regulates bone sialoprotein gene transcription." in: Journal of cellular biochemistry, Vol. 113, Issue 9, pp. 2822-34, (2012) (PubMed).

Niu, Kondo, Nakazawa, Oishi, Kawasaki, Mochizuki, Yamane, Katoh: "Transcription factor Runx2 is a regulator of epithelial-mesenchymal transition and invasion in thyroid carcinomas." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 92, Issue 8, pp. 1181-90, (2012) (PubMed).

Wang, Li, Li, Yang, Sasaki, Wang, Zhou, Araki, Mezawa, Takai, Ogata: "Effects of inorganic polyphosphate on bone sialoprotein gene expression." in: Gene, Vol. 452, Issue 2, pp. 79-86, (2010) (PubMed).

Li, Sasaki, Mezawa, Wang, Li, Yang, Wang, Zhou, Araki, Matsumura, Takai, Ogata: "cAMP and fibroblast growth factor 2 regulate bone sialoprotein gene expression in human prostate cancer cells." in: Gene, Vol. 471, Issue 1-2, pp. 1-12, (2010) (PubMed).

Target Details for RUNX2

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Alternative Name: RUNX2 (RUNX2 Products)

Background: Full Gene Name: runt-related transcription factor 2
Synonyms: AML3,CBFA1,CCD,CCD1,MGC120022,MGC120023,OSF2,PEA2aA,PEBP2A1,PEBP2A2,PEBP2aA,PEBP2aA1

Gene ID: 860

NCBI Accession: NM_004348