GRAP2 antibody

Catalog No. ABIN509570

Product Details anti-GRAP2 Antibody

Target: GRAP2 (GRB2-Related Adaptor Protein 2 (GRAP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRAP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody UW40 recognizes GRAP2/GADS, a 41 kDa cytoplasmic adaptor protein that plays a pivotal role during the early events of signal transduction in T cells.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: GST-fusion human GRAP2/GADS protein

Clone: UW40

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: Recommended dilution: 2-6 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-GRAP2 antibody (ABIN509570)

Yankee, Yun, Draves, Ganesh, Bevan, Murali-Krishna, Clark: "The Gads (GrpL) adaptor protein regulates T cell homeostasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 3, pp. 1711-20, (2004) (PubMed).

Yankee, Draves, Ewings, Clark, Graves: "CD95/Fas induces cleavage of the GrpL/Gads adaptor and desensitization of antigen receptor signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 12, pp. 6789-93, (2001) (PubMed).

Target Details for GRAP2

Target: GRAP2 (GRB2-Related Adaptor Protein 2 (GRAP2))

Alternative Name: GRAP2/GADS (GRAP2 Products)

Synonyms: GADS antibody, GRAP-2 antibody, GRB2L antibody, GRBLG antibody, GRID antibody, GRPL antibody, GrbX antibody, Grf40 antibody, Mona antibody, P38 antibody, Gads antibody, GrpL antibody, zgc:112091 antibody, GRAP2 antibody, grap2 antibody, zgc:65942 antibody, GRB2-related adaptor protein 2 antibody, GRB2-related adaptor protein 2a antibody, GRB2-related adaptor protein 2b antibody, GRAP2 antibody, Grap2 antibody, grap2a antibody, grap2 antibody, grap2b antibody

Background: GRB2 related adaptor protein 2,GRAP2/GADS (Grb2-related adaptor protein 2 / Grb2-related adaptor downstream of Shc) is a cytoplasmic adaptor protein containing N- and C-terminal SH3 domains flanking a central SH2 domain and a proline/glutamine-rich region. It is expressed predominantly in lymphoid tissue and hematopoietic cells, particularly in T cells. GRAP2/GADS plays a pivotal role during the early events of T cell signal transduction by recruiting the adaptor protein SLP-76 and its associated molecules, such as Vav, Nck, Itk, and ADAP, to the transmembrane adaptor protein LAT. GRAP2/GADS also binds several other signaling proteins, namely Gab2, HPK1 (hematopoietic progenitor kinase 1), and Cbl. Unlike similar adaptor protein Grb2, GRAP2/GADS shows higher selectivity when binding to the particular phosphorylated tyrosines of LAT adaptor.,GADS, GRID, GRAP-2, GrbX

Gene ID: 9402

UniProt: O75791

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway