Sulfotyrosine antibody (HRP)

Catalog No. ABIN5067500

Product Details anti-Sulfotyrosine Antibody (HRP)

Target: Sulfotyrosine

Host: Mouse

Clonality: Monoclonal

Conjugate: This Sulfotyrosine antibody is conjugated to HRP

Application: ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.

Purification: Protein G Purified

Immunogen: Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).

Clone: 7C5

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:50)
• ELISA (1:1000)
• IHC (1:25)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5067500 was sufficient for detection of Sulfotyrosine in 0.5 μg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Sulfotyrosine

Target: Sulfotyrosine

Abstract: Sulfotyrosine Products

Target Type: Amino Acid

Background: Tyrosine sulfation is a common post-translational protein modification catalyzed by tyrosylprotein sulfotransferase (TPST) (1). Sulfotyrosines mediate interactions between hirudin and thrombin, P-selectin glycoprotein ligand-1 and P-selectin, and von Willebrand factor and factor VIII. Tyrosine sulfation of certain receptors is required for several viruses and parasites to enter their hosts.