Sulfotyrosine antibody (Atto 488)
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- Target See all Sulfotyrosine products
- Sulfotyrosine
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Sulfotyrosine antibody is conjugated to Atto 488
- Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
- Specificity
- Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.
- Purification
- Protein G Purified
- Immunogen
- Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
- Clone
- 7C5
- Isotype
- IgG1
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- Application Notes
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- WB (1:1000)
- ICC/IF (1:50)
- ELISA (1:1000)
- IHC (1:25)
- optimal dilutions for assays should be determined by the user.
- Comment
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A 1:1000 dilution of ABIN5067489 was sufficient for detection of Sulfotyrosine in 0.5 μg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- Sulfotyrosine
- Abstract
- Sulfotyrosine Products
- Target Type
- Amino Acid
- Background
- Tyrosine sulfation is a common post-translational protein modification catalyzed by tyrosylprotein sulfotransferase (TPST) (1). Sulfotyrosines mediate interactions between hirudin and thrombin, P-selectin glycoprotein ligand-1 and P-selectin, and von Willebrand factor and factor VIII. Tyrosine sulfation of certain receptors is required for several viruses and parasites to enter their hosts.
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