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Malondialdehyde antibody (HRP)

MDA WB, ELISA, ICC, IF, IHC Host: Mouse Monoclonal 11E3 HRP
Catalog No. ABIN5067374
  • Target See all Malondialdehyde (MDA) Antibodies
    Malondialdehyde (MDA)
    Reactivity
    Please inquire
    Host
    • 22
    • 2
    • 1
    Mouse
    Clonality
    • 22
    • 3
    Monoclonal
    Conjugate
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This Malondialdehyde antibody is conjugated to HRP
    Application
    • 23
    • 21
    • 21
    • 20
    • 13
    • 10
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
    Specificity
    Specific for MDA conjugated proteins. Does not detect free MDA. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-HHE, 4-HNE, or Methylglyoxal modified proteins.
    Purification
    Protein G Purified
    Immunogen
    Synthetic Malondialdehyde modified Keyhole Limpet Hemocyanin (KLH).
    Clone
    11E3
    Isotype
    IgG1
    Top Product
    Discover our top product MDA Primary Antibody
  • Application Notes
    • WB (1:1000)
    • ICC/IF (1:50)
    • ELISA (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Comment

    A 1:1000 dilution of ABIN5067374 was sufficient for detection of Malondialdehyde in 2 μg of Malondialdehyde conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    Malondialdehyde (MDA)
    Alternative Name
    Malondialdehyde (MDA Products)
    Target Type
    Chemical
    Background
    Malondialdehyde (MDA) is the biomarker in greatest diagnostic use, due to its molecular stability. This three-carbon, low-molecular weight aldehyde has a strong affinity for amino acids, which results in adduct formation to both free amino acids and proteins. Increased MDA levels have been found at correlating levels in breast cancer, and lung cancer patients. Other diseased states with elevated MDA levels include diabetes and Alzheimer's disease. Multiple laboratory techniques exist for quantification of MDA levels, including the thiobarbituric acid reactive substances (TBARS) assay. In addition to use as a biomarker, MDA has been shown to have mutagenic effects on tissues themselves as adduct formation can result in DNA cross-linking.
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