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- Target See all HNE Antibodies
- HNE (4-Hydroxynonenal (HNE))
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This HNE antibody is conjugated to FITC
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Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificity
- Specific for 4-HNE modified proteins. Does not detect free 4-Hydroxynonenal. Does not X-react with 4-HHE, Acrolein, Crotonaldehyde, Hexanoyl Lysine, MDA, or Methylglyoxal modified proteins.
- Purification
- Protein G Purified
- Immunogen
- Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
- Clone
- 12F7
- Isotype
- IgG1
- Top Product
- Discover our top product HNE Primary Antibody
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anti-4-Hydroxynonenal (HNE) antibody
HNE WB, ELISA, IF (p), IHC (fro), IHC (p) Host: Rabbit Polyclonal unconjugated
anti-4-Hydroxynonenal (HNE) antibodyHNE WB, ELISA, IHC Host: Mouse Monoclonal HNEJ-2 unconjugated
anti-4-Hydroxynonenal (HNE) antibodyHNE WB, ELISA, ICC, IF Host: Mouse Monoclonal 12F7 unconjugated
anti-4-Hydroxynonenal (HNE) antibody (HRP)HNE WB, ELISA, ICC, IF Host: Mouse Monoclonal 12F7 HRP
anti-4-Hydroxynonenal (HNE) antibody (Biotin)HNE WB, ELISA, ICC, IF Host: Mouse Monoclonal 12F7 Biotin
anti-4-Hydroxynonenal (HNE) antibody (PE)HNE WB, ELISA, ICC, IF Host: Mouse Monoclonal 12F7 PE
anti-4-Hydroxynonenal (HNE) antibody (PerCP)HNE WB, ELISA, ICC, IF Host: Mouse Monoclonal 12F7 PerCP
anti-4-Hydroxynonenal (HNE) antibody (APC)HNE WB, ELISA, ICC, IF Host: Mouse Monoclonal 12F7 APC
anti-4-Hydroxynonenal (HNE) antibody (Atto 594)HNE WB, ELISA, ICC, IF Host: Mouse Monoclonal 12F7 Atto 594
anti-4-Hydroxynonenal (HNE) antibody (Atto 488)HNE WB, ELISA, ICC, IF Host: Mouse Monoclonal 12F7 Atto 488
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- Application Notes
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- WB (1:1000)
- ICC/IF (1:50)
- ELISA (1:1000)
- optimal dilutions for assays should be determined by the user.
- Comment
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A 1:1000 dilution of ABIN5067301 was sufficient for detection of 4-Hydroxynonenal in 0.5 μg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- HNE (4-Hydroxynonenal (HNE))
- Alternative Name
- 4-Hydroxynonenal (HNE Products)
- Target Type
- Chemical
- Background
- 4-Hydroxy-2-nonenol (4-HNE) is an unsaturated aldehyde derived from lipid peroxidation. 4-HNE is an electrophile and reacts with protein nucleophiles such as cysteine, histine, and lysine (1). Low levels of 4-HNE promote cell survival via cellular antioxidant induction whereas higher levels lead to autophagy, apoptosis, and ultimately necrosis. 4-HNE has been linked to Alzheimer's disease, Parkinson's disease, cancer, cardiovascular diseases, diabetes, and liver disease.
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