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HNE antibody (APC)

HNE WB, ELISA, ICC, IF Host: Mouse Monoclonal 12F7 APC
Catalog No. ABIN5067299
  • Target See all HNE Antibodies
    HNE (4-Hydroxynonenal (HNE))
    Host
    • 15
    • 11
    • 1
    Mouse
    Clonality
    • 16
    • 11
    Monoclonal
    Conjugate
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HNE antibody is conjugated to APC
    Application
    • 26
    • 13
    • 13
    • 9
    • 9
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
    Specificity
    Specific for 4-HNE modified proteins. Does not detect free 4-Hydroxynonenal. Does not X-react with 4-HHE, Acrolein, Crotonaldehyde, Hexanoyl Lysine, MDA, or Methylglyoxal modified proteins.
    Purification
    Protein G Purified
    Immunogen
    Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
    Clone
    12F7
    Isotype
    IgG1
    Top Product
    Discover our top product HNE Primary Antibody
  • Application Notes
    • WB (1:1000)
    • ICC/IF (1:50)
    • ELISA (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Comment

    A 1:1000 dilution of ABIN5067299 was sufficient for detection of 4-Hydroxynonenal in 0.5 μg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    HNE (4-Hydroxynonenal (HNE))
    Alternative Name
    4-Hydroxynonenal (HNE Products)
    Target Type
    Chemical
    Background
    4-Hydroxy-2-nonenol (4-HNE) is an unsaturated aldehyde derived from lipid peroxidation. 4-HNE is an electrophile and reacts with protein nucleophiles such as cysteine, histine, and lysine (1). Low levels of 4-HNE promote cell survival via cellular antioxidant induction whereas higher levels lead to autophagy, apoptosis, and ultimately necrosis. 4-HNE has been linked to Alzheimer's disease, Parkinson's disease, cancer, cardiovascular diseases, diabetes, and liver disease.
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