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Acrolein antibody (Biotin)

ACR ELISA, FACS, WB, ICC, IF, IHC Host: Mouse Monoclonal 10A10 Biotin
Catalog No. ABIN5067192
  • Target See all Acrolein (ACR) products
    Acrolein (ACR)
    Host
    • 9
    Mouse
    Clonality
    • 9
    Monoclonal
    Conjugate
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Acrolein antibody is conjugated to Biotin
    Application
    ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
    Specificity
    Specific for Acrolein modified proteins. Does not detect free acrolein. Does not X-react with Crotonaldehyde, Hexanoyl Lysine, 4-HHE, 4-HNE, MDA, or Methylglyoxal modified proteins.
    Purification
    Protein G Purified
    Immunogen
    Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).
    Clone
    10A10
    Isotype
    IgG1
  • Application Notes
    • WB (1:1000)
    • ICC/IF (1:50)
    • FACS (1:50)
    • FCM (1:50)
    • ELISA (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Comment

    A 1:1000 dilution of ABIN5067192 was sufficient for detection of Acrolein in 2 μg of Acrolein conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    Acrolein (ACR)
    Alternative Name
    Acrolein (ACR Products)
    Target Type
    Chemical
    Background
    Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Acrolein is an electrophilic conjugated aldehyde that is a terminal product of lipid peroxidation. Acrolein is highly mutagenic and reacts with nucleophilic functional groups in DNA and proteins such as cysteine, histidine, and lysine residues (1).
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