Acrolein antibody (Biotin)
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- Target See all Acrolein (ACR) products
- Acrolein (ACR)
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Acrolein antibody is conjugated to Biotin
- Application
- ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
- Specificity
- Specific for Acrolein modified proteins. Does not detect free acrolein. Does not X-react with Crotonaldehyde, Hexanoyl Lysine, 4-HHE, 4-HNE, MDA, or Methylglyoxal modified proteins.
- Purification
- Protein G Purified
- Immunogen
- Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).
- Clone
- 10A10
- Isotype
- IgG1
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- Application Notes
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- WB (1:1000)
- ICC/IF (1:50)
- FACS (1:50)
- FCM (1:50)
- ELISA (1:1000)
- optimal dilutions for assays should be determined by the user.
- Comment
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A 1:1000 dilution of ABIN5067192 was sufficient for detection of Acrolein in 2 μg of Acrolein conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- Acrolein (ACR)
- Alternative Name
- Acrolein (ACR Products)
- Target Type
- Chemical
- Background
- Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Acrolein is an electrophilic conjugated aldehyde that is a terminal product of lipid peroxidation. Acrolein is highly mutagenic and reacts with nucleophilic functional groups in DNA and proteins such as cysteine, histidine, and lysine residues (1).
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