Arginylation (N-Term) antibody (PE)
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- Target
- Arginylation
- Binding Specificity
- N-Term
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- PE
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Application
- Western Blotting (WB), Flow Cytometry (FACS)
- Specificity
- Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.
- Purification
- Protein G Purified
- Immunogen
- Synthetic N-terminal arginylated peptide conjugated to KLH
- Clone
- 4A9
- Isotype
- IgG1
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- Application Notes
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- WB (1:1000)
- FCM (1:50)
- optimal dilutions for assays should be determined by the user.
- Comment
-
A 1:1000 dilution of ABIN5066981 was sufficient for detection of N-terminal Arginylation in 0.5 ug of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- Arginylation
- Target Type
- Chemical
- Background
- Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood (1). Arginylation has also been shown to regulate cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress (2).
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