ATG4A antibody (AA 102-113) (FITC)

Catalog No. ABIN5065987

Product Details anti-ATG4A Antibody (FITC)

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Binding Specificity: AA 102-113

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG4A antibody is conjugated to FITC

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.,Detects ~45 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Peptide Affinity Purified

Immunogen: Synthetic peptide from the mid-protein of Human ATG4A (aa. 102-113)

Isotype: IgG

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5065987 was sufficient for detection of ATG4A in 15 μg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for ATG4A

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Alternative Name: ATG4A (ATG4A Products)

Synonyms: apg4a antibody, autl2 antibody, F6E13.27 antibody, APG4A antibody, zgc:111958 antibody, Aut2a antibody, AUTL2 antibody, AI627006 antibody, AV169859 antibody, Apg4a antibody, Atg4al antibody, Autl2 antibody, Atg4a antibody, autophagy related 4A cysteine peptidase L homeolog antibody, autophagy related 4A cysteine peptidase antibody, Peptidase family C54 protein antibody, ATG4 autophagy related 4 homolog A (S. cerevisiae) antibody, autophagy related 4A, cysteine peptidase antibody, atg4a.L antibody, ATG4A antibody, AT2G44140 antibody, atg4a antibody, Atg4a antibody

Background: Autophagy related 4A, cysteine peptidase (ATG4A or APG4A) is a C-54 cysteine protease that also belongs to the family of autophagin proteins, and is primarily found in skeletal muscle and brain. ATG4A is involved in cellular autophagy and has been studied as a target for cancer therapy. Specifically it cleaves GATE-16. Mutations in the ATG4a gene is critical for redox regulation and inhibits autophagosome formation.

Gene ID: 115201

NCBI Accession: NP_001308216

UniProt: Q8WYN0

Pathways: Autophagy