MERS-Coronavirus Spike antibody

Catalog No. ABIN5027657

Product Details anti-MERS-Coronavirus Spike Antibody

Target: MERS-Coronavirus Spike (MERS-CoV S) (MERS-Coronavirus Spike Protein (MERS-CoV S))

Reactivity: Middle East Respiratory Syndrome Coronavirus (MERS-CoV)

Host: Mouse

Clonality: Monoclonal

Conjugate: This MERS-Coronavirus Spike antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Protein G Chromatography

Immunogen: A recombinant protein fragment of MERS-CoV Spike (S) protein was used as the immunogen for this antibody.

Clone: ABM4A80

Isotype: IgG2b kappa

Application Details

Application Notes: WB: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Target Details for MERS-Coronavirus Spike

Target: MERS-Coronavirus Spike (MERS-CoV S) (MERS-Coronavirus Spike Protein (MERS-CoV S))

Alternative Name: MERS-CoV Spike (S) protein (MERS-CoV S Products)

Target Type: Viral Protein

Background: The MERS-CoV spike (S) protein is a type I transmembrane glycoprotein which contains 1353 amino acids and can be cleaved into two subunit S1 and S2. It forms large protruding spikes on the surface of the virus. The S1 subunit which contains RBD is responsible for binding to Dipeptidyl peptidase 4, which serves as the host cell receptor of MERS-CoV and S2 mediates the membrane fusion. In this process, heptad repeats 1 and 2 (HR1 and HR2) of the S protein assemble into a complex called six-helix bundle (6-HB) fusion core structure, which represents a key membrane fusion architecture. Importantly, the S protein is considered a key component of vaccines against coronavirus infection, including severe acute respiratory syndrome (SARS).

Molecular Weight: 28 kDa

UniProt: W5ZZU6