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MFN1 antibody (AA 1-234)

MFN1 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN5013951
  • Target See all MFN1 Antibodies
    MFN1 (Mitofusin 1 (MFN1))
    Binding Specificity
    • 16
    • 7
    • 7
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-234
    Reactivity
    • 51
    • 30
    • 27
    • 21
    • 16
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 61
    • 5
    • 3
    • 1
    Rabbit
    Clonality
    • 58
    • 12
    Polyclonal
    Conjugate
    • 33
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MFN1 antibody is un-conjugated
    Application
    • 53
    • 26
    • 15
    • 11
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Purpose
    Polyclonal Antibody to Mitofusin 1 (MFN1)
    Specificity
    The antibody is a rabbit polyclonal antibody raised against MFN1. It has been selected for its ability to recognize MFN1 in immunohistochemical staining and western blotting.
    Cross-Reactivity
    Human, Rat
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    Recombinant Mitofusin 1 (MFN1) corresdonding to Met1~Ile234
    Isotype
    IgG
    Top Product
    Discover our top product MFN1 Primary Antibody
  • Application Notes

    Western blotting: 0.5-2 μg/mL

    1:500-2000 Immunohistochemistry: 5-20 μg/mL

    1:50-200 Immunocytochemistry: 5-20 μg/mL

    1:50-200 Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Validation #103013 (Western Blotting)
    'Independent Validation' Badge
    by
    Wang Lab, Department of Animal Science, College of Agriculture & Natural Resources
    No.
    #103013
    Date
    05/30/2018
    Antigen
    MFN1
    Lot Number
    A20180411903
    Method validated
    Western Blotting
    Positive Control

    293T cells transfected with MFN1-HA expression vector; the full length MFN1 CDS has been inserted upstream of an HA-tag in plasmid pcDNA3.1.

    Negative Control

    293T cells transfected with an LUC-HA expression vector, and the full length LUC CDS has been inserted upstream of an HA-tag in plasmid pcDNA3.1.

    293T cells transfected with MFN2-HA expression vector; the full length MFN2 CDS has been inserted upstream of an FLAG-tag in plasmid pcDNA3.1.

    Notes

    Passed. ABIN5013951 specifically recognizes antigen in sample.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN5013951
    Secondary Antibody
    goat anti-rabbit IgG (H+L) antibody HRP conjugate (Bio-Rad, 170-6515)
    Full Protocol
    • Grow 293T cells in DMEM medium (Gibco, lot 1897400) supplemented with 10% fetal bovine serum (Gibco, lot 1913756) and 1% Penicillin-Streptomycin (thermo, 10378016), at 37°C and 7% CO2 in 6-well plates to 60-70% confluency.
    • Transfect cells with plasmids expressing either MFN1-HA, MFN2-HA or LUC-HA with max 2µg of plasmid using PEI transfection reagent (Poly-Science, 24765-1) following the manufacturer´s instructions.
    • Lyse cells in 100µl RIPA buffer containing protease inhibitors (Sigma-Aldrich, P8340).
    • Determine total protein content of the lysates using a Bradford protein assay (Protein Assay Dye Reagent Concentrate, Bio-Rad, 5000006).
    • Denature 100µg total protein for 10min at 95°C in 1x SDS loading buffer and subsequently separate them on a freshly cast 10% denaturing polyacrylamide gel.
    • Transfer proteins onto Immun-Blot Low Fluorescence PVDF Membrane (0.45μm) at 1.0A and up to 25V for 30min via Trans-Blot Turbo Transfer System.
    • Block the membrane with Bio-rad Blotting-Grade Blocker (5% non-fat milk in PBS).
    • Incubation with primary
      • rabbit anti-MFN1 antibody (antibodies-online, ABIN5013951, lot A20180411903) diluted 1:200 in Blocking Buffer (5% non-fat milk) ON at 4°C.
      • mouse anti-HA-tag antibody (Santa Cruz, sc-7392) diluted 1:2000 in Blocking Buffer (5% non-fat milk) ON at 4°C (only LUC-HA expressing cells).
      • mouse anti-MFN2-tag antibody (Santa Cruz, sc-100560) diluted 1:2000 in Blocking Buffer (5% non-fat milk) ON at 4°C.
      • mouse anti-beta-actin antibody (Sigma-Aldrich, A1978) diluted 1:10000 in Blocking Buffer(5% non-fat milk) ON at 4°C.
    • Wash membrane 3x for 10min with PBS supplemented with 0.1% Tween 20.
    • Incubation with secondary goat anti-rabbit IgG (H+L) antibody HRP conjugate (Bio-Rad, 170-6515) or goat anti-mouse IgG (H+L) antibody HRP conjugate (Bio-Rad, 170-6516) diluted 1:10000 in Odyssey Blocking Buffer for 1h at RT.
    • Wash membrane 3x for 10min with PBS supplemented with 0.1% Tween 20.
    • Add Clarity and Clarity Max Western ECL Blotting Substrates and scan the membrane on a Bio-Rad ChemiDoc XRS+System.
    Experimental Notes

    The antigen antibody ABIN5013951 reveals a protein of the expected molecular weight of antigen in lysates of cells. The protein bands is only visible in the positive but not the negative controls.

  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Preservative
    ProClin
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    MFN1 (Mitofusin 1 (MFN1))
    Alternative Name
    Mitofusin 1 (MFN1 Products)
    Synonyms
    mfn1 antibody, zgc:66150 antibody, mitofusin-1 antibody, MFN1 antibody, MFN2 antibody, hfzo1 antibody, hfzo2 antibody, 2310002F04Rik antibody, 6330416C07Rik antibody, D3Ertd265e antibody, HR2 antibody, mKIAA4032 antibody, Fzo1b antibody, mitofusin 1b antibody, mitofusin 1 antibody, mitofusin 1 S homeolog antibody, mfn1b antibody, MFN1 antibody, mfn1.S antibody, mfn1 antibody, Mfn1 antibody
    Background
    Hfzo1, Hfzo2, Transmembrane GTPase MFN1, Fzo homolog
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