TSEN2 antibody (AbBy Fluor® 750)

Catalog No. ABIN5012025

Product Details anti-TSEN2 Antibody (AbBy Fluor® 750)

Target: TSEN2 (tRNA-splicing Endonuclease Subunit SEN2 (TSEN2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TSEN2 antibody is conjugated to AbBy Fluor® 750

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TSEN2

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TSEN2

Target: TSEN2 (tRNA-splicing Endonuclease Subunit SEN2 (TSEN2))

Alternative Name: TSEN2 (TSEN2 Products)

Synonyms: AU067695 antibody, RGD1309946 antibody, PCH2B antibody, SEN2 antibody, SEN2L antibody, tRNA splicing endonuclease subunit 2 antibody, TSEN2 antibody, Tsen2 antibody

Background:

Synonyms: HsSen2, MGC2776, MGC4440, SEN2, SEN2L, tRNA intron endonuclease Sen2, tRNA splicing endonuclease 2 homolog SEN2, S. cerevisiae, tRNA splicing endonuclease subunit Sen2, TSEN 2, SEN2_HUMAN.

Background: The tRNA-splicing endonuclease complex is responsible for identifying and cleaving pre-tRNA at both 5? and 3? splice sites, thereby releasing introns and free tRNA molecules with 2',3' cyclic phosphates and 5'-OH termini. In addition to its role in pre-tRNA splicing, the heterotetrameric endonuclease complex participates in mRNA processing and, via its association with pre-mRNA processing factors, is thought to link pre-tRNA and pre-mRNA splicing events. TSEN2 (tRNA-splicing endonuclease subunit Sen2), also known as tRNA-intron endonuclease Sen2, is a 465 amino acid nuclear protein that constitutes one of the two catalytic subunits of the tRNA-splicing endonuclease complex. There are three isoforms of TSEN2 that are produced as a result of alternative splicing events. Isoform 1 seems to carry the active site for 5?-splice site cleavage. Defects in the gene encoding TSEN2 are the cause of pontocerebellar hypoplasia type 2B, which is characterized by progressive microencephaly with epilepsy, extrapyramidal dyskinesia and chorea without spinal cord findings.

Gene ID: 80746

Pathways: Nuclear Receptor Transcription Pathway, Regulation of Lipid Metabolism by PPARalpha