STIM1 antibody (C-Term)

Catalog No. ABIN500827

Product Details anti-STIM1 Antibody

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STIM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects STIM1 / GOK. It is predicted to have no cross-reactivity to STIM2.

Purification: Peptide affinity chromatography

Immunogen: STIM1 antibody was raised against a 24 amino acid peptide from near the carboxy terminus of human STIM1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunohisochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-STIM1 antibody (ABIN500827)

Wu, Hsu, Wang, Liang, Tsai, Yen, Li, Chiu, Chang, Huang, Lin, Yoshioka: "A Novel Strategy for TNF-Alpha Production by 2-APB Induced Downregulated SOCE and Upregulated HSP70 in O. tsutsugamushi-Infected Human Macrophages." in: PLoS ONE, Vol. 11, Issue 7, pp. e0159299, (2017) (PubMed).

Misceo, Holmgren, Louch, Holme, Mizobuchi, Morales, De Paula, Stray-Pedersen, Lyle, Dalhus, Christensen, Stormorken, Tjønnfjord, Frengen: "A dominant STIM1 mutation causes Stormorken syndrome." in: Human mutation, Vol. 35, Issue 5, pp. 556-64, (2014) (PubMed).

Target Details for STIM1

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Alternative Name: STIM1 / GOK (STIM1 Products)

Synonyms: stim1 antibody, MGC76045 antibody, STIM1 antibody, si:dkey-24p1.5 antibody, MGC154748 antibody, si:ch211-112l17.2 antibody, Stim1 antibody, D11S4896E antibody, GOK antibody, TAM antibody, SIM antibody, stromal interaction molecule 1 antibody, stromal interaction molecule 1a antibody, stromal interaction molecule 1 L homeolog antibody, stromal interaction molecule 1b antibody, STIM1 antibody, stim1 antibody, stim1a antibody, stim1.L antibody, stim1b antibody, Stim1 antibody

Background: In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least two isoforms of STIM1 are known to exist, this antibody will detect only the larger form.Synonyms: Stromal interaction molecule 1

Gene ID: 6786

NCBI Accession: NP_003147

Pathways: TCR Signaling, BCR Signaling