SPRED3 antibody (Center)

Catalog No. ABIN500802

Product Details anti-SPRED3 Antibody

Target: SPRED3 (Sprouty-Related, EVH1 Domain Containing 3 (SPRED3))

Binding Specificity: Center

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPRED3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects Spred3 at center. It is predicted to have no cross-reactivity to Spred1 or Spred2.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Peptide affinity chromatography

Immunogen: Spred3 antibody was raised against a 16 amino acid peptide near the center of the human Spred3.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 2 - 4 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for SPRED3

Target: SPRED3 (Sprouty-Related, EVH1 Domain Containing 3 (SPRED3))

Alternative Name: Spred3 (SPRED3 Products)

Synonyms: si:ch211-255l12.2 antibody, RGD1311713 antibody, Eve-3 antibody, spred-3 antibody, AW047143 antibody, D130060H24Rik antibody, Spred-3 antibody, sprouty related EVH1 domain containing 3 antibody, sprouty-related, EVH1 domain containing 3 antibody, SPRED3 antibody, spred3 antibody, Spred3 antibody

Background: Spred3 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1 and Spred2, Spred3 contains and EVH1 domain in its amino terminus and a Sprouty-related cysteine-rich (SPR) domain in its C-terminus, but does not possess a functional c-kit binding domain (KBD). The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Although Spred3 was initially reported to be expressed in brain, at least one report also indicates that Spred3 can be found in liver. At least two isoforms of Spred3 are known to exist.Synonyms: EVE-3, Sprouty-related EVH1 domain-containing protein 3

Gene ID: 399473

NCBI Accession: NP_001035987