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NLE1 antibody (AA 351-450) (AbBy Fluor® 680)

NLE1 Reactivity: Mouse WB, IF (cc), IF (p) Host: Rabbit Polyclonal AbBy Fluor® 680
Catalog No. ABIN5007676
  • Target See all NLE1 Antibodies
    NLE1 (Notchless Homolog 1 (NLE1))
    Binding Specificity
    • 14
    • 10
    • 8
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 351-450
    Reactivity
    • 40
    • 28
    • 13
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 54
    Rabbit
    Clonality
    • 54
    Polyclonal
    Conjugate
    • 20
    • 5
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NLE1 antibody is conjugated to AbBy Fluor® 680
    Application
    • 41
    • 26
    • 12
    • 12
    • 4
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    Cross-Reactivity
    Mouse
    Predicted Reactivity
    Human,Rat,Cow,Sheep,Pig,Horse,Rabbit
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human NLE1
    Isotype
    IgG
    Top Product
    Discover our top product NLE1 Primary Antibody
  • Application Notes
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    NLE1 (Notchless Homolog 1 (NLE1))
    Alternative Name
    NLE1 (NLE1 Products)
    Synonyms
    Nle antibody, AL022765 antibody, BC018399 antibody, l11Jus1 antibody, l11Jus4 antibody, RGD1308301 antibody, notchless homolog 1 antibody, notchless homolog 1 (Drosophila) antibody, NLE1 antibody, Nle1 antibody
    Background

    Synonyms: FLJ10458, NLE 1, Nle, Notchless gene homolog, Notchless homolog 1 Drosophila, Notchless homolog 1, Notchless protein homolog 1, NLE1_HUMAN.

    Background: The Notch signaling pathway is an evolutionary conserved system that is involved in intracellular communication. Notch receptors play an important role in development and cell-fate decisions. Notchless is a loss-of-function mutant allele that encodes for protein NLE1 (notchless homolog 1). NLE1 is a 485 amino acid WD40-repeat protein that binds to the cytoplasmic domain of Notch, regulating its signaling activity in Drosophila melanogaster and in mice. Deletion of the NLE1 gene in mice during the early stages of development results in embryonic death, while gene deletion in the late stages of development leads to activation of a caspase-3-dependent apoptotic pathway. In plants, NLE1 is crucial for normal cellular growth and development. Under-expression during shoot proliferation causes pleiotropic defects such as delayed flowering and abnormal organ maturation. It may also play a role in 60S ribosomal subunit biogenesis in yeast. NLE1 contains eight WD40 domains and produces one isoform due to alternative splicing.

    Gene ID
    54475
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