UIMC1 antibody (N-Term)

Catalog No. ABIN500569

Product Details anti-UIMC1 Antibody

Target: UIMC1 (Ubiquitin Interaction Motif Containing 1 (UIMC1))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UIMC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects UIMC1 / RAP80 at N-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: RAP80 antibody was raised against a 17 amino acid peptide from near the amino terminus of human RAP80.

Isotype: IgG

Application Details

Application Notes: ELISA: Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for UIMC1

Target: UIMC1 (Ubiquitin Interaction Motif Containing 1 (UIMC1))

Alternative Name: UIMC1 / RAP80 (UIMC1 Products)

Synonyms: UIMC1 antibody, RAP80 antibody, X2HRIP110 antibody, 9430016E08Rik antibody, D330018D10Rik antibody, D630032M02Rik antibody, RIP110 antibody, Rxrip110 antibody, RGD1307009 antibody, ubiquitin interaction motif containing 1 antibody, UIMC1 antibody, Uimc1 antibody

Background: RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.Synonyms: BRCA1-A complex subunit RAP80, RXRIP110, Receptor-associated protein 80, Retinoid X receptor-interacting protein 110, Ubiquitin interaction motif-containing protein 1

Gene ID: 51720

UniProt: Q96RL1

Pathways: DNA Damage Repair, Nuclear Hormone Receptor Binding, Positive Regulation of Response to DNA Damage Stimulus