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ISR-beta antibody (pTyr1355) (AbBy Fluor® 680)

ISR-beta Reactivity: Human, Rat, Mouse WB, FACS, IF (cc), IF (p) Host: Rabbit Polyclonal AbBy Fluor® 680
Catalog No. ABIN5005454
  • Target See all ISR-beta Antibodies
    ISR-beta (Insulin Receptor beta (ISR-beta))
    Binding Specificity
    • 16
    • 16
    • 15
    • 15
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr1355
    Reactivity
    • 78
    • 72
    • 57
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    Human, Rat, Mouse
    Host
    • 75
    • 3
    Rabbit
    Clonality
    • 75
    • 3
    Polyclonal
    Conjugate
    • 12
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    This ISR-beta antibody is conjugated to AbBy Fluor® 680
    Application
    • 59
    • 47
    • 38
    • 38
    • 16
    • 10
    • 10
    • 6
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic phosphopeptide derived from human Insulin Receptor beta around the phosphorylation site of Tyr1355 [RS(p-Y)EE]
    Isotype
    IgG
    Top Product
    Discover our top product ISR-beta Primary Antibody
  • Application Notes
    FCM 1:20-100
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    ISR-beta (Insulin Receptor beta (ISR-beta))
    Alternative Name
    Insulin Receptor Beta (ISR-beta Products)
    Synonyms
    CD220 antibody, HHF5 antibody, 4932439J01Rik antibody, D630014A15Rik antibody, IR antibody, IR-A antibody, IR-B antibody, insulin receptor antibody, INSR antibody, Insr antibody
    Background

    Synonyms: HHF5, CD220, Insulin receptor, IR, INSR

    Background: Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. Binding of the SH2 domains of PI3K to phosphotyrosines on IRS1 leads to the activation of PI3K and the generation of phosphatidylinositol-(3, 4, 5)-triphosphate (PIP3), a lipid second messenger, which activates several PIP3-dependent serine/threonine kinases, such as PDPK1 and subsequently AKT/PKB. The net effect of this pathway is to produce a translocation of the glucose transporter SLC2A4/GLUT4 from cytoplasmic vesicles to the cell membrane to facilitate glucose transport. Moreover, upon insulin stimulation, activated AKT/PKB is responsible for: anti-apoptotic effect of insulin by inducing phosphorylation of BAD, regulates the expression of gluconeogenic and lipogenic enzymes by controlling the activity of the winged helix or forkhead (FOX) class of transcription factors. Another pathway regulated by PI3K-AKT/PKB activation is mTORC1 signaling pathway which regulates cell growth and metabolism and integrates signals from insulin. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 thereby activating mTORC1 pathway.

    Gene ID
    3643
    UniProt
    P06213
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