PAK2 antibody (N-Term)

Catalog No. ABIN500441

Product Details anti-PAK2 Antibody

Target: PAK2 (P21-Activated Kinase 2 (PAK2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects PAK2 at N-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: 14 amino acid peptide from near the amino terminus of human PAK2

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

References for anti-PAK2 antibody (ABIN500441)

Choi, Pease, Chen, Zhang, Phee: "P21-activated kinase 2 is essential in maintenance of peripheral Foxp3+ regulatory T cells." in: Immunology, Vol. 154, Issue 2, pp. 309-321, (2018) (PubMed).

Phee, Au-Yeung, Pryshchep, OHagan, Fairbairn, Radu, Kosoff, Mollenauer, Cheng, Chernoff, Weiss: "Pak2 is required for actin cytoskeleton remodeling, TCR signaling, and normal thymocyte development and maturation." in: eLife, Vol. 3, pp. e02270, (2015) (PubMed).

Target Details for PAK2

Target: PAK2 (P21-Activated Kinase 2 (PAK2))

Alternative Name: PAK2 (PAK2 Products)

Synonyms: PAK65 antibody, PAKgamma antibody, gamma-PAK antibody, 5330420P17Rik antibody, A130002K10Rik antibody, AI836325 antibody, D16Ertd269e antibody, PAK-2 antibody, mKIAA4182 antibody, pak2 antibody, cb422 antibody, kinase antibody, zgc:91798 antibody, wu:fb71h01 antibody, si:dz198m22.1 antibody, pak65 antibody, pakgamma antibody, PAKI antibody, Pak3 antibody, CH73-159L23.1 antibody, wu:fc52b03 antibody, zgc:113857 antibody, p21 (RAC1) activated kinase 2 antibody, p21 protein (Cdc42/Rac)-activated kinase 2 antibody, p21 protein (Cdc42/Rac)-activated kinase 2a antibody, p21 protein (Cdc42/Rac)-activated kinase 2 S homeolog antibody, p21 protein (Cdc42/Rac)-activated kinase 2b antibody, PAK2 antibody, Pak2 antibody, pak2a antibody, pak2.S antibody, pak2b antibody

Background: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6, and are not activated by Cdc42/Rac binding. Of the six PAK proteins, only PAK2 is ubiquitously expressed and cleaved by caspase-3. This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment. Recent experiments have shown that following cleavage, the active fragment is myristoylated and directed to the plasma membrane and membrane ruffles where it promotes cell death via increased signaling through the c-Jun N-terminal kinase pathway, but without compromising mitochondrial integrity.Synonyms: Gamma-PAK, PAK 2, PAK gamma, PAK-2, PAK65, Serine/threonine-protein kinase PAK 2, p21-activated kinase 2, p58

Gene ID: 5062

NCBI Accession: NP_002568

UniProt: Q13177

Pathways: MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha