Nicastrin antibody (Center)

Catalog No. ABIN500345

Product Details anti-Nicastrin Antibody

Target: Nicastrin (NCSTN)

Binding Specificity: Center

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nicastrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects Nicastrin at center.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: Nicastrin antibody was raised against a 18 amino acid peptide from near the center of human Nicastrin.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for Nicastrin

Target: Nicastrin (NCSTN)

Alternative Name: Nicastrin (NCSTN Products)

Synonyms: CG7012 antibody, Dmel\\CG7012 antibody, Nct antibody, Nic antibody, S5A antibody, ST96B antibody, agro antibody, dNct antibody, nic antibody, NCSTN antibody, DKFZp469O2418 antibody, APH2 antibody, 9430068N19Rik antibody, AA727311 antibody, Aph2 antibody, D1Dau13e antibody, Kiaa0253 antibody, mKIAA0253 antibody, nicastrin antibody, Nicastrin antibody, NCSTN antibody, nct antibody, ncstn antibody, CpipJ_CPIJ001617 antibody, Ncstn antibody, aph-2 antibody

Background: Nicastrin, in addition to presenilin, PEN2, and APH-1 forms the γ-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-a-converting enzyme (TACE) and the b-site cleavage enzyme (BACE) protease families, γ-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the α and γ cleavage site) or the toxic Aβ amyloid peptide (from the β and γ cleavage site). It is thought that accumulation of the Aβ peptide is the precursor to Alzheimer's disease. Nicastrin is also thought to be involved in cell proliferation and signaling, especially in regards to activation of Notch receptors as loss of Nicastrin expression results in mouse embryonic lethality.Synonyms: KIAA0253, NCSTN, UNQ1874/PRO4317

Gene ID: 23385

NCBI Accession: NP_056146

UniProt: Q92542

Pathways: Notch Signaling, Neurotrophin Signaling Pathway