NLRP1 antibody (C-Term)

Catalog No. ABIN500310

Product Details anti-NLRP1 Antibody

Target: NLRP1 (NLR Family, Pyrin Domain Containing 1 (NLRP1))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NLRP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: This antibody detects CARD7 / NLRP1.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Peptide affinity chromatography

Immunogen: NALP1 antibody was raised against a peptide corresponding to 13 amino acids near the carboxy-terminus of human NALP1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for NLRP1

Target: NLRP1 (NLR Family, Pyrin Domain Containing 1 (NLRP1))

Alternative Name: CARD7 / NLRP1 (NLRP1 Products)

Synonyms: CARD7 antibody, CIDED antibody, CLR17.1 antibody, DEFCAP antibody, DEFCAP-L/S antibody, NAC antibody, NALP1 antibody, PP1044 antibody, SLEV1 antibody, VAMAS1 antibody, NLR family pyrin domain containing 1 antibody, NLRP1 antibody, Nlrp1 antibody

Background: NALP1 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli (1). It contains a caspase recruitment domain (CARD) in addition to a NACHT domain and leucine rich repeat (LRR). This protein interacts strongly with caspase 2 and weakly with caspase 9, as well as with Apaf-1 (2). Transient overexpression of this gene in cultured cells was sufficient to induce apoptosis (3). NALP1 mRNA and protein expression levels in myeloid leukemia cells are increased following CREB (cAMP-response element binding protein) activation, suggesting that NALP1 may contribute to modulate the response of these cells to pro-inflammatory stimuli (4). Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for NALP1 (3).Synonyms: Caspase recruitment domain-containing protein 7, DEFCAP, Death effector filament-forming ced-4-like apoptosis protein, KIAA0926, LRR and PYD domains-containing protein 1, NAC, NACHT, NALP1, Nucleotide-binding domain and caspase recruitment domain

Gene ID: 22861

UniProt: Q9C000

Pathways: Positive Regulation of Endopeptidase Activity, Inflammasome