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MED28 antibody (Center)

MED28 Reactivity: Human, Mouse WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN500268
  • Target See all MED28 Antibodies
    MED28 (Mediator Complex Subunit 28 (MED28))
    Binding Specificity
    • 15
    • 5
    • 1
    • 1
    • 1
    • 1
    Center
    Reactivity
    • 19
    • 17
    • 3
    • 1
    • 1
    Human, Mouse
    Host
    • 32
    Rabbit
    Clonality
    • 32
    Polyclonal
    Conjugate
    • 8
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MED28 antibody is un-conjugated
    Application
    • 13
    • 13
    • 9
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificity
    This antibody detects MED28.
    Cross-Reactivity (Details)
    Species reactivity (tested):Human, mouse
    Purification
    Peptide affinity chromatography
    Immunogen
    MED28 antibody was raised against a 17 amino acid peptide from near the center of human MED28.
    Isotype
    IgG
    Top Product
    Discover our top product MED28 Primary Antibody
  • Application Notes
    ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Buffer
    PBS containing 0.02 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
  • Target
    MED28 (Mediator Complex Subunit 28 (MED28))
    Alternative Name
    MED28 (MED28 Products)
    Synonyms
    1500003D12Rik antibody, EG1 antibody, magicin antibody, Eg1 antibody, FKSG20 antibody, RGD1305875 antibody, wu:fc74b11 antibody, zgc:103633 antibody, AI451633 antibody, AU045690 antibody, mediator complex subunit 28 antibody, MED28 antibody, Med28 antibody, med28 antibody
    Background
    The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED28, also known as Magicin. MED28 is expressed in many cell lines and tissues. It has been shown that a down-regulation of MED28 expression in NIH3T3 cells results in a significant induction of several genes associated with smooth muscle cell differentiation and conversely its over-expression represses expression of SMC genes. MED28 can also form a ternary complex with Grb2 and Merlin, the neurofibromatosis 2 tumor suppressor protein, indicating that MED28 may play a role in Merlin's tumor suppressive activity. MED28 has also been recently identified as an HIV dependency factor (HDF), suggesting that MED28 may be an important drug target in HIV treatment. At least two isoforms of MED28 are known to exist.Synonyms: EG1, Endothelial-derived protein 1, FKSG20, Mediator complex subunit 28, Mediator of RNA polymerase II transcription subunit 28, Merlin and Grb2-interacting cytoskeletal protein, Tumor angiogenesis marker EG-1
    Gene ID
    80306
    NCBI Accession
    NP_079481
    UniProt
    Q9H204
    Pathways
    Stem Cell Maintenance, Regulation of Muscle Cell Differentiation
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