JMJD1C antibody (N-Term)

Catalog No. ABIN500078

Product Details anti-JMJD1C Antibody

Target: JMJD1C (Jumonji Domain Containing 1C (JMJD1C))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JMJD1C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects JMJD1C / TRIP8. It will not cross-react with JMJD1A or JMJD1B.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: JMJD1C antibody was raised against a 20 amino acid peptide from near the amino terminus of human JMJD1C.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for JMJD1C

Target: JMJD1C (Jumonji Domain Containing 1C (JMJD1C))

Alternative Name: JMJD1C / TRIP8 (JMJD1C Products)

Background: The jumonji domain containing 1C protein (JMJD1C) was initially discovered in silico, and later suggested to be a candidate gene for autism. Like the related proteins JMJD1A and JMJD1B, JMJD1C is a histone H3K9 demethylase implicated in the nuclear hormone receptor-based transcriptional regulation. JMJD1C mRNA is highly expressed in undifferentiated embryonic stem (ES) cells as well as pancreatic islet, diffuse-type gastric cancer, and other tissues and tumors. The JMJD1C gene promoter contain bHLH-, AP-1-, and POU5F1-binding sites, and as preferential expression of POU5F1 has been reported in ES cells, pancreatic islet, and diffuse-type gastric cancer, it has been suggested that POU5F1-mediated expression of JMJD1C reactivates previously silenced genes in ES cells and diffuse-type gastric cancer. At least three isoforms of JMJD1C are known to exist.Synonyms: JHDM2C, Jumonji domain-containing protein 1C, KIAA1380, Probable JmjC domain-containing histone demethylation protein 2C, Thyroid receptor-interacting protein 8

Gene ID: 221037

NCBI Accession: NP_116165

UniProt: Q15652

Pathways: Nuclear Hormone Receptor Binding