DARC antibody (N-Term)

Catalog No. ABIN499712

Product Details anti-DARC Antibody

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DARC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to DARC.

Purification: Affinity chromatography purified via peptide column

Immunogen: DARC antibody was raised against a 18 amino acid peptide from near the amino terminus of human DARC.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 1 - 2 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for DARC

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Alternative Name: CD234 / DARC (DARC Products)

Synonyms: DARC antibody, FY antibody, ACKR1 antibody, CCBP1 antibody, CD234 antibody, Dfy antibody, GPD antibody, GpFy antibody, WBCQ1 antibody, AA162249 antibody, Ackr1 antibody, ESTM35 antibody, Duffy blood group, chemokine receptor antibody, atypical chemokine receptor 1 (Duffy blood group) antibody, atypical chemokine receptor 1 antibody, DARC antibody, ACKR1 antibody, Ackr1 antibody

Background: DARC, also known as the Duffy antigen/chemokine receptor, is a seven-transmembrane protein homologous to the classical chemokine G-protein coupled receptors (GPCRs) with the exception of the motif required for G protein coupling. DARC can bind with high affinity several chemokines without transducing any signal, suggesting it may modulate the signals normally induced by these chemokines. Recently, DARC was found to interact with KAI1, a four transmembrane protein recently identified as a tumor metastasis suppressor protein. It is thought that tumor cells dislodged from the primary tumor and expressing KAI1 interact with DARC proteins expressed on vascular cells, transmitting a senescent signal to the tumor cells, while tumor cells that have lost KAI1 expression can proliferate and potentially give rise to metastases. At least three isoforms of DARC are known to exist.Synonyms: Duffy antigen/chemokine receptor, FY, Fy glycoprotein, GPD, Glycoprotein D, GpFy, Plasmodium vivax receptor

Gene ID: 2532

UniProt: Q16570