Caspase 4 antibody (N-Term)

Catalog No. ABIN499561

Product Details anti-Caspase 4 Antibody

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: This antibody was raised against a 16 amino acid peptide from the amino-terminus of human Caspase-4.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human Casp-4 / ICH-2 / ICE rel II / Mih1 (N-Terminus) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: Casp-4 antibody can be used for the detection of Caspase-4 at 1 μg/mL. Depending on cell lines or tissues used, other cleavage products may be observed. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Caspase 4

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Alternative Name: Caspase-4 (CASP4 Products)

Synonyms: ICE(rel)II antibody, ICEREL-II antibody, ICH-2 antibody, Mih1/TX antibody, TX antibody, Casp11 antibody, CASP13 antibody, CASP1 antibody, CASP4 antibody, Caspl antibody, ich-3 antibody, Casp4 antibody, caspase 4 antibody, caspase 4, apoptosis-related cysteine peptidase antibody, caspase-4 antibody, CASP4 antibody, Casp4 antibody, LOC100731032 antibody

Background: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1). Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1beta and IL-18 (2,3). Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells (4). More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimer's disease (5).Synonyms: CASP4, ICE(rel)-II, ICH2, Protease ICH-2, Protease TX

Gene ID: 837

UniProt: P49662

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity