BORA antibody (N-Term)

Catalog No. ABIN499491

Product Details anti-BORA Antibody

Target: BORA (Protein Aurora Borealis (BORA))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BORA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to BORA.

Purification: Affinity chromatography purified via peptide column

Immunogen: Bora antibody was raised against a 15 amino acid peptide from near the amino terminus of human Bora.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: Bora antibody can be used for detection of Bora at 1-2 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for BORA

Target: BORA (Protein Aurora Borealis (BORA))

Alternative Name: Protein Aurora Borealis (BORA Products)

Synonyms: CG6897 antibody, Dmel\\CG6897 antibody, zgc:101616 antibody, C13orf34 antibody, 6720463M24Rik antibody, AI317232 antibody, RGD1309522 antibody, c13orf34 antibody, C12H13orf34 antibody, aurora borealis antibody, bora, aurora kinase A activator antibody, protein aurora borealis antibody, bora, aurora kinase A activator S homeolog antibody, bora antibody, Tsp_06066 antibody, Bora antibody, BORA antibody, bora.S antibody

Background: Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.Synonyms: BORA, C13orf34, FLJ22624, HsBora

Gene ID: 79866

UniProt: Q6PGQ7