BCAS2 antibody (C-Term)

Catalog No. ABIN499423

Product Details anti-BCAS2 Antibody

Target: BCAS2 (Breast Carcinoma Amplified Sequence 2 (BCAS2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCAS2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Purification: Affinity chromatography purified via peptide column

Immunogen: BCAS2 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human BCAS2.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: BCAS2 antibody can be used for detection of BCAS2 at 0.5 - 1 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for BCAS2

Target: BCAS2 (Breast Carcinoma Amplified Sequence 2 (BCAS2))

Alternative Name: BCAS2 / DAM1 (BCAS2 Products)

Synonyms: DAM1 antibody, SPF27 antibody, Snt309 antibody, 6430539P16Rik antibody, AI132645 antibody, C76366 antibody, C80030 antibody, BCAS2 antibody, cb302 antibody, zgc:101730 antibody, BCAS2, pre-mRNA processing factor antibody, breast carcinoma amplified sequence 2 antibody, breast carcinoma amplified sequence 2 L homeolog antibody, BCAS2 antibody, Bcas2 antibody, bcas2 antibody, bcas2.L antibody, CpipJ_CPIJ003889 antibody

Background: BCAS2 was identified through differential display analysis as an mRNA species that was overexpressed in MCF7 and BT-20 breast cancer cell lines. The chromosomal region containing this gene, 1p13.3021, is amplified in these cells lines. BCAS2 is a transcriptional cofactor that enhances estrogen receptor-mediated gene expression, and directly interacts with the tumor suppressor p53 to reduce p53 transcriptional activity by reducing p53 protein level in the absence of DNA damage. Deprivation of BCAS2 through RNA inhibition induces apoptosis in p53-wild type cells, but causes G2-M arrest in p53-null or -mutant cells, this effect was reversed with the expression of ectopic BCAS2. BCAS2 may thus be potentially useful as a therapeutic target in the treatment of cancer.Synonyms: Breast carcinoma-amplified sequence 2, DNA amplified in mammary carcinoma 1 protein, Pre-mRNA-splicing factor SPF27, SPF27

Gene ID: 10286

UniProt: O75934