AP2M1 antibody (C-Term)

Catalog No. ABIN499285

Product Details anti-AP2M1 Antibody

Target: AP2M1 (Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Chicken

Clonality: Polyclonal

Conjugate: This AP2M1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to AP2M1.

Purification: Affinity chromatography purified via peptide column

Immunogen: AP2M1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human AP2M1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: AP2M1 antibody can be used for detection of AP2M1 at 1 - 2 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for AP2M1

Target: AP2M1 (Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1))

Abstract: AP2M1 Products

Background: The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA)-4 protein. At least two isoforms of AP2M1 are known to exist.Synonyms: AP-2 mu-2 chain, Adaptin-mu2, CLAPM1, Clathrin assembly protein complex 2 medium chain, Clathrin coat assembly protein AP50, Clathrin coat-associated protein AP5, HA2 50 kDa subunit, KIAA0109, Plasma membrane adaptor AP-2 50 kDa protein

Gene ID: 1173

NCBI Accession: NP_004059

UniProt: Q96CW1

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation, SARS-CoV-2 Protein Interactome