TUBA1A antibody (N-Term)

Catalog No. ABIN499269

Product Details anti-TUBA1A Antibody

Target: TUBA1A (Tuba1a) (Tubulin, alpha 1a (Tuba1a))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Chicken

Clonality: Polyclonal

Conjugate: This TUBA1A antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to Alpha-tubulin.

Purification: Affinity chromatography purified via peptide column

Immunogen: Tubulin antibody was raised against a 16 amino acid peptide near the amino terminus of human Tubulin.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: Tubulin antibody can be used for detection of Tubulin at 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

References for anti-TUBA1A antibody (ABIN499269)

Ambadipudi, Georges: "Sequences in Linker-1 domain of the multidrug resistance associated protein (MRP1 or ABCC1) bind to tubulin and their binding is modulated by phosphorylation." in: Biochemical and biophysical research communications, Vol. 482, Issue 4, pp. 1001-1006, (2017) (PubMed).

Schmid, Sailland, Novak, Baumlin, Fregien, Salathe: "Modulation of Wnt signaling is essential for the differentiation of ciliated epithelial cells in human airways." in: FEBS letters, Vol. 591, Issue 21, pp. 3493-3506, (2017) (PubMed).

Target Details for TUBA1A

Target: TUBA1A (Tuba1a) (Tubulin, alpha 1a (Tuba1a))

Alternative Name: alpha Tubulin / TUBA1A / TUBA3 (Tuba1a Products)

Background: Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. Like GAPDH and beta-Actin, this antibody makes an excellent loading control in immunoblots.Synonyms: Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-1A chain, Tubulin alpha-3 chain

Gene ID: 7846

UniProt: Q71U36

Pathways: Microtubule Dynamics, M Phase