ACIN1 antibody (Intermediate Domain)

Catalog No. ABIN499201

Product Details anti-ACIN1 Antibody

Target: ACIN1 (Apoptotic Chromatin Condensation Inducer 1 (ACIN1))

Binding Specificity: Intermediate Domain

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACIN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: Acinus antibody was raised against a peptide corresponding to amino acids 775 to 789 of Human AcinusL, 48 to 62 of human AcinusS', or 17 to 31 of human AcinusS, which differ from those of mouse Acinus by one amino acid .

Purification: Affinity chromatography purified via peptide column

Immunogen: Human Acinus (Intermediate Domain) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: Acinus antibody can be used for detection of Acinus at 0.5 to 1 μg/mL. A 220 kDa band can be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ACIN1

Target: ACIN1 (Apoptotic Chromatin Condensation Inducer 1 (ACIN1))

Alternative Name: Acinus / ACIN1 (ACIN1 Products)

Background: Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE . A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS (2).Synonyms: Apoptotic chromatin condensation inducer in the nucleus, KIAA0670

Gene ID: 22985

UniProt: Q9UKV3