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AAK1 antibody (N-Term)

AAK1 Reactivity: Human, Mouse, Rat WB, IF, EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN499183
  • Target See all AAK1 Antibodies
    AAK1 (AP2 associated kinase 1 (AAK1))
    Binding Specificity
    • 15
    • 9
    • 8
    • 6
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 42
    • 38
    • 12
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 62
    • 4
    • 1
    Rabbit
    Clonality
    • 64
    • 3
    Polyclonal
    Conjugate
    • 33
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This AAK1 antibody is un-conjugated
    Application
    • 42
    • 30
    • 20
    • 13
    • 13
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
    Specificity
    This antibody recognizes AAK1 near the amino terminus.
    Purification
    Affinity chromatography purified via peptide column
    Immunogen
    A 20 amino acid peptide near the amino terminus of the uman Aak1
    Isotype
    IgG
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    Discover our top product AAK1 Primary Antibody
  • Application Notes
    ELISA. Western Blot: Aak1 antibody can be used for detection of Aak1 at 1 - 2 μg/mL. Immunofluorescence.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Buffer
    PBS containing 0.02 % Sodium Azide as preservative
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    AAK1 (AP2 associated kinase 1 (AAK1))
    Alternative Name
    AAK1 (AAK1 Products)
    Synonyms
    5530400K14Rik antibody, 9630042K20 antibody, AU067724 antibody, AU067726 antibody, BC028270 antibody, C79663 antibody, D6Ertd245e antibody, R75501 antibody, mKIAA1048 antibody, RGD1563580 antibody, aak1 antibody, si:dkey-261l11.5 antibody, AP2 associated kinase 1 antibody, uncharacterized protein FLJ45252 antibody, AP2 associated kinase 1b antibody, AAK1 antibody, Aak1 antibody, LOC100512341 antibody, aak1b antibody
    Background
    AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxf) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist, the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.Synonyms: AP2-associated protein kinase 1, KIAA1048
    Gene ID
    22848
    NCBI Accession
    NP_055726
    UniProt
    Q2M2I8
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