TIAL1 antibody (Internal Region)

Catalog No. ABIN499121

Product Details anti-TIAL1 Antibody

Target: TIAL1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein-Like 1 (TIAL1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This TIAL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-SPEITTEDIK SAFAP

Specificity: This antibody is expected to recognize both isoforms (NP_003243.1, NP_001029097.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Canine, Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the Internal region of Human TIAL1 (NP_003243.1 and NP_001029097.1) Genename: TIAL1

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/64000. Western blot: 1-3 μg/mL. Approx 45 kDa band observed in lysates of cell line K562.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TIAL1

Target: TIAL1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein-Like 1 (TIAL1))

Alternative Name: TIAL1 (TIAL1 Products)

Background: TIA1 is a member of an RNA binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15- kDa protein that is thought to be derived from the carboxyl terminus of the 40- kDa product by proteolytic processing.Synonyms: Nucleolysin TIAR, TIA-1-related protein

Molecular Weight: 43.4 kDa (NP_001029097.1)

Gene ID: 7073

NCBI Accession: NP_003243

UniProt: Q01085