×
For best experience we recommend to activate Javascript in your browser.
MDFI antibody
MDFI
Reactivity: Human, Mouse
WB
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-MDFI Antibody
(hide)
Target
See all MDFI Antibodies
MDFI
(MyoD Family Inhibitor (MDFI))
Reactivity
All reactivities for MDFI antibodies
Human, Mouse
Host
All hosts for MDFI antibodies
Rabbit
Clonality
All clonalities for MDFI antibodies
Polyclonal
Conjugate
All conjugates for MDFI antibodies
This MDFI antibody is un-conjugated
Application
All applications for MDFI antibodies
Western Blotting (WB)
Specificity
This antibody detects endogenous levels of MDFI protein. (region surrounding Pro134)
Cross-Reactivity (Details)
Species reactivity (expected):Mouse. Species reactivity (tested):Human.
Purification
Affinity chromatography using epitope-specific immunogen
Purity
> 95 % pure by SDS-PAGE
Top Product
Discover our top product MDFI Primary Antibody
Alternatives
(show)
Application Details
(hide)
Application Notes
Western blot: 1: 500 - 1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
(hide)
Concentration
1.0 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Target Details for MDFI
(hide)
Target
MDFI
(MyoD Family Inhibitor (MDFI))
Alternative Name
MDFI (MDFI Products )
Synonyms
MDFI antibody, I-MF antibody, I-mfa antibody, I-mf antibody, RGD1560271 antibody, MyoD family inhibitor antibody, MDFI antibody, Mdfi antibody
Background
MDFI is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. MDFI an also interfere with the DNA-binding activity of Myod family members and plays an important role in trophoblast and chondrogenic differentiation.Synonyms: MyoD family inhibitor
Molecular Weight
approx. 25 kDa
Gene ID
4188
NCBI Accession
NP_005577
UniProt
Q99750
Pathways
Maintenance of Protein Location
Recently viewed
(hide)