PFKL antibody
-
- Target See all PFKL Antibodies
- PFKL (Phosphofructokinase, Liver (PFKL))
-
Reactivity
- Human, Mouse, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This PFKL antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- Specificity
- This antibody detects endogenous levels of PFK-C protein. (region surroudning Gln365)
- Cross-Reactivity (Details)
-
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- Affinity chromatography using epitope-specific immunogen
- Purity
- > 95 % (by SDS-PAGE)
- Top Product
- Discover our top product PFKL Primary Antibody
-
-
- Application Notes
-
Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
-
- Target
- PFKL (Phosphofructokinase, Liver (PFKL))
- Alternative Name
- PFKL (PFKL Products)
- Synonyms
- PFK-B antibody, AA407869 antibody, phosphofructokinase, liver type antibody, phosphofructokinase, liver, B-type antibody, PFKL antibody, Pfkl antibody
- Background
- The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle and liver isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.Synonyms: 6-phosphofructokinase liver, PFK-B, Phosphofructokinase 1, Phosphohexokinase
- Molecular Weight
- approx. 85 kDa
- Gene ID
- 5211
- NCBI Accession
- NP_002617
- UniProt
- P17858
- Pathways
- Negative Regulation of Hormone Secretion, Warburg Effect
-