Dystroglycan antibody (Internal Region)
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- Target See all Dystroglycan (DAG1) Antibodies
- Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse, Rat, Cow, Dog
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Dystroglycan antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Sequence
- C-HVGKHEYFMH ATDK
- Specificity
- This antibody is expected to recognize both the precursor and the mature Alpha-Dystroglycan, but not the mature Beta-Dystroglycan.
- Cross-Reactivity (Details)
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Species reactivity (expected):Canine (Dog), Bovine.
Species reactivity (tested):Human, Mouse, Rat. - Purification
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
- Immunogen
- Peptide from the internal region of the protein sequence according to NP_004384.3. Genename: DAG1
- Top Product
- Discover our top product DAG1 Primary Antibody
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- Application Notes
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Peptide ELISA: Detection Limit: 1/128000. Western blot: 0.2-0.6 μg/mL. Detects a band of Approx 100 kDa in Human Skeletal Muscle lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))
- Alternative Name
- Dystroglycan (DAG1 Products)
- Synonyms
- LOC398500 antibody, dag1 antibody, MGC53537 antibody, 156DAG antibody, A3a antibody, AGRNR antibody, DAG antibody, MDDGC7 antibody, MDDGC9 antibody, DAG1 antibody, RAB7 antibody, dg antibody, a3a antibody, dag antibody, agrnr antibody, 156dag antibody, D9Wsu13e antibody, DG antibody, Dp427 antibody, Dp71 antibody, CG18250 antibody, CT41273 antibody, DmDG antibody, Dmel\\CG18250 antibody, atu antibody, dgn antibody, dys antibody, GB14967 antibody, APOJ antibody, CLI antibody, RATTRPM2B antibody, SGP-2 antibody, SGP2 antibody, SP-40 antibody, SP40 antibody, TRPM-2 antibody, TRPM2B antibody, Trpm2 antibody, Trpmb antibody, Ala-1 antibody, H9/25 antibody, Ly-27 antibody, Ly-6 antibody, Ly27 antibody, wu:fb83d06 antibody, wu:fi25f06 antibody, wu:fi37b08 antibody, zgc:109786 antibody, DystroGlycaN antibody, dystroglycan 1 L homeolog antibody, dystroglycan 1 antibody, dystroglycan 1 S homeolog antibody, Dystroglycan antibody, dystroglycan antibody, clusterin antibody, lymphocyte antigen 6 complex antibody, dgn-1 antibody, dag1.L antibody, dgn-2 antibody, dgn-3 antibody, DAG1 antibody, dag1.S antibody, Dag1 antibody, dag1 antibody, Dg antibody, LOC408826 antibody, Clu antibody, Ly6 antibody
- Background
- Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha Dystroglycan and a 43 kDa transmembrane protein, beta Dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin.Synonyms: DAG1, Dystrophin-associated glycoprotein 1
- Gene ID
- 1605
- NCBI Accession
- NP_004384
- UniProt
- Q14118
- Pathways
- Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus
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