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ACPP antibody (Internal Region)
ACPP
Reactivity: Human, Mouse, Rat, Cow, Dog
WB, IHC (p), EIA
Host: Goat
Polyclonal
unconjugated
Product Details anti-ACPP Antibody
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Target
See all ACPP Antibodies
ACPP
(Acid Phosphatase, Prostate (ACPP))
Binding Specificity
All epitopes for ACPP antibodies
Internal Region
Reactivity
All reactivities for ACPP antibodies
Human, Mouse, Rat, Cow, Dog
Host
All hosts for ACPP antibodies
Goat
Clonality
All clonalities for ACPP antibodies
Polyclonal
Conjugate
All conjugates for ACPP antibodies
This ACPP antibody is un-conjugated
Application
All applications for ACPP antibodies
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
Sequence
YGIHKQKEKS RLQ
Specificity
This antibody is expected to recognize both isoforms (NP_001090.2, NP_001127666.1).
Cross-Reactivity (Details)
Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine. Species reactivity (tested):Human.
Purification
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Immunogen
Peptide from the internal region of Human ACPP according to NP_001090.2. Genename: ACPP
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Alternatives
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Application Details
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Application Notes
Peptide ELISA: Detection Limit: 1/64000. Western blot: 0.3-1 μg/mL. Detects a band of Approx 50 kDa in Human Prostate lysates. The observed molecular weightcorresponds to earlier findings in literature with different antibodies (Patel et al. , ClinChem.1986 Oct,32(10): 1832-5. PMID: 3530530). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
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Concentration
0.5 mg/mL
Buffer
Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target Details for ACPP
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Target
ACPP
(Acid Phosphatase, Prostate (ACPP))
Alternative Name
ACPP / Prostatic Acid Phosphatase (ACPP Products )
Synonyms
5'-NT antibody, ACP-3 antibody, ACP3 antibody, PAP antibody, AMAP2 antibody, CENTB3 antibody, DDEF2 antibody, PAG3 antibody, Pap-alpha antibody, SHAG1 antibody, A030005E02Rik antibody, FRAP antibody, Lap antibody, Ppal antibody, Acpp11 antibody, RNACPP11 antibody, pap antibody, ACPP antibody, acp-3 antibody, acp3 antibody, acpt antibody, acid phosphatase, prostate antibody, ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 antibody, prostatic acid phosphatase antibody, prostatic acid phosphatase, putative antibody, acid phosphatase, prostate S homeolog antibody, ACPP antibody, ASAP2 antibody, Acpp antibody, CpipJ_CPIJ004002 antibody, Smp_016640 antibody, acpp.S antibody
Background
Prostatic acid phosphatase is one of the two antigenic markers of prostatic carcinoma, the other being prostate specific antigen. It belongs to the kallikrein family of serine proteases and is suggested to act as a hydrolase to split phospharyl choline in semen and as a transferase.Synonyms: ACP3, PAP, PSAP, Prostate acid phosphatase
Molecular Weight
44.6kDa (NP_001090.2)
Gene ID
55
NCBI Accession
NP_001090
UniProt
P15309
Pathways
Synaptic Membrane , Ribonucleoside Biosynthetic Process
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