ELISA. Western blot: Beclin-1 antibody can be used for the detection of Beclin-1 at 0.5-1 μg/mL. Positive Control: 293 Cell Lysate. Immunohistochemistry on Paraffin Sections. Positive Control: Rat Brain Tissue. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
PBS containing 0.02 % Sodium Azide as preservative
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store undiluted at 2-8 °C. Antibodies should not be exposed to prolonged high temperatures.
Target
Beclin 1 (BECN1)
(Beclin 1, Autophagy Related (BECN1))
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells (1,2). Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6 (3,4), is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors (5). Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death (6).Synonyms: BECN1, Coiled-coil myosin-like BCL2-interacting protein, GT197, Protein GT197