HY5 antibody
HY5
Reactivity: Arabidopsis thaliana
WB
Host: Rabbit
Polyclonal
unconjugated
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- Target See all HY5 products
- HY5 (Transcription Factor HY5 (HY5))
- Reactivity
- Arabidopsis thaliana
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB)
- Cross-Reactivity (Details)
- Not reactive in: Oryza sativa, Pisum sativum, Populus sp.
- Predicted Reactivity
- Brassica pekinensis
- Immunogen
- KLH-conjugated peptide, derived from Arabidopsis thaliana HY5 protein sequence, UniProt:O24646 , TAIR: AT5G11260. Chosen peptide is not conserved in HYH protein sequence.
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- Application Notes
- 1: 1000 (WB)
- Comment
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This antibody detects well recombinant HY5 in Nicotiana benthamiana (image below) while a signal in endogenous total cell extract of Arabidopsis thaliana has not been optimised in applied conditions (as below).Using nuclear fraction for detection of endogenous protein is recommened, however it has not been optimized yet.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please add 50 μL of sterile water For reconstitution
- Storage
- -20 °C
- Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Target
- HY5 (Transcription Factor HY5 (HY5))
- Alternative Name
- Protein long hypocotyl 5 (HY5) (HY5 Products)
- Synonyms
- BZIP TRANSCRIPTION FACTOR HY5 antibody, ELONGATED HYPOCOTYL 5 antibody, F2I11.150 antibody, F2I11_150 antibody, REVERSAL OF THE DET PHENOTYPE 5 antibody, TED 5 antibody, Basic-leucine zipper (bZIP) transcription factor family protein antibody, HY5 antibody
- Background
- HY5 (Protein long hypocotyl 5) is a light-regulated transcription factor that promotes photomorphogenesis in light. Involved in the blue light specific pathway. Heterodimer. Expressed in root, hypocotyl, cotyledon, leaf, stem and floral organs. Localized in nucleus. Alternative names: Protein LONG HYPOCOTYL 5, bZIP transcription factor 56, AtbZIP56.
- Molecular Weight
- 18.5 kDa
- UniProt
- O24646
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