Abscisic Acid Insensitive 2 (ABI2) (N-Term) antibody
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- Target
- Abscisic Acid Insensitive 2 (ABI2)
- Binding Specificity
- N-Term
- Reactivity
- Arabidopsis thaliana
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB)
- Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- Immunogen
- KLH-conjugated synthetic peptide derived from N-terminus of Arabidopsis thaliana ABI2 sequence, UniProt: O04719, ,TAIR: AT5G57050 chosen peptide is not conserved in ABI1
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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ABI2 antibodies recognize recombiant StrepTag-ABI2, ABI2-GST, His-ABI2. ABI2 protein is easily degraded therefore extraction buffer needs to contain protease inhibotors, example of such inhibitor coctail can be found here.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 μL of sterile water.
- Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Target
- Abscisic Acid Insensitive 2 (ABI2)
- Background
- Protein Abscisic acid-insensitive 2 (ABI2) is a represor of the ABA signaling pathway, regulating various ABA responses like: stomatal closure, osmotic water permability of the plasma membrane, high light stress, response to glucose, seed germination and inhibition of vegetative growth. Involved in acquired thermotolerance. Alternative names: ABA INSENSITIVE 2, ABI2, ATABI2, Protein phosphatase 2C 77, AtPP2C77, At5g57050, MHM17_19, MHM17.19, PP2C ABI2.
- Molecular Weight
- 46 kDa
- UniProt
- O04719
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