WT1 antibody (AA 346-375)
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- Target See all WT1 Antibodies
- WT1 (Wilms Tumor 1 (WT1))
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Binding Specificity
- AA 346-375
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This WT1 antibody is un-conjugated
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), ELISA
- Cross-Reactivity (Details)
- Expected species reactivity: Mouse, Rat, Pig, Xenopus
- Purification
- Antigen affinity purified
- Immunogen
- A portion of amino acids 346-375 from the human protein was used as the immunogen for this WT1 antibody.
- Isotype
- Ig Fraction
- Top Product
- Discover our top product WT1 Primary Antibody
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anti-Wilms Tumor 1 (WT1) (AA 1-181) antibody
WT1 Reactivity: Human FACS, IHC, WB, ELISA, ICC Host: Mouse Monoclonal 7F9E8 unconjugated
anti-Wilms Tumor 1 (WT1) (AA 1-302) antibodyWT1 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-Wilms Tumor 1 (WT1) (AA 346-375) antibodyWT1 Reactivity: Human, Mouse IF, FACS, WB, IHC (p) Host: Rabbit Polyclonal RB18841 unconjugated
anti-Wilms Tumor 1 (WT1) (AA 1-181) antibodyWT1 Reactivity: Human FACS, WB, ELISA Host: Mouse Monoclonal 7F9G7H2 unconjugated
anti-Wilms Tumor 1 (WT1) antibodyWT1 Reactivity: Human, Mouse, Rat IHC, StM Host: Mouse Monoclonal rWT1-857 unconjugated Recombinant Antibody
anti-Wilms Tumor 1 (WT1) (AA 314-479) antibodyWT1 Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 5G11A5 unconjugated
anti-Wilms Tumor 1 (WT1) (AA 1-181) antibodyWT1 Reactivity: Human, Mouse, Rat IHC, StM Host: Mouse Monoclonal 6F-H2 unconjugated
anti-Wilms Tumor 1 (WT1) (C-Term) antibodyWT1 Reactivity: Human, Mouse, Rat, Rabbit, Dog, Bat, Chicken, Monkey, Xenopus laevis IHC, WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Wilms Tumor 1 (WT1) (AA 1-302) antibodyWT1 Reactivity: Human IF, WB Host: Rabbit Polyclonal unconjugated
anti-Wilms Tumor 1 (WT1) (Middle Region) antibodyWT1 Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Pig, Zebrafish (Danio rerio), Horse IHC, WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
- Titration of the WT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot the WT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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- Target
- WT1 (Wilms Tumor 1 (WT1))
- Alternative Name
- WT1 (Wilms Tumor Protein) (WT1 Products)
- Background
- Wilms Tumor protein is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq].
- UniProt
- P19544
- Pathways
- Tube Formation
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