WEE1 antibody (AA 202-230)

Catalog No. ABIN3029617

Product Details anti-WEE1 Antibody

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Binding Specificity: AA 202-230

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WEE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Expected species reactivity: Rat

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 202-230 from the mouse protein was used as the immunogen for this Wee1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Wee1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Wee1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for WEE1

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Alternative Name: Wee1 (WEE1 Products)

Synonyms: WEE1A antibody, WEE1hu antibody, ATWEE1 antibody, F22D16.3 antibody, F22D16_3 antibody, WEE1 kinase homolog antibody, WEE1-KINASE antibody, WEE1 antibody, Xe-Wee1B antibody, XeWee1B antibody, wee1-b antibody, wee1a antibody, wee2-a antibody, xe-wee1a antibody, xwee1 antibody, wee1 antibody, wee1b antibody, wee1hu antibody, xwee2 antibody, GB19689 antibody, Wee1A antibody, fa20h03 antibody, wu:fa20h03 antibody, zgc:136514 antibody, WEE1 G2 checkpoint kinase antibody, Wee1 kinase antibody, WEE1-like kinase antibody, WEE1 G2 checkpoint kinase S homeolog antibody, wee1 homolog antibody, WEE1 homolog 2 antibody, wee1-like protein kinase antibody, WEE 1 homolog 1 (S. pombe) antibody, M phase inhibitor protein kinase Wee1 antibody, WEE1 antibody, Wee1 antibody, wee1.S antibody, wee1-a antibody, wee2 antibody, LOC551677 antibody, wee1 antibody

Background: Wee1 may act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur (By similarity).

UniProt: P47810

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase