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V5 Epitope Tag antibody

Name: V5 Epitope Tag antibody
SKU: ABIN3029565
Price: 649.42 USD
Availability: InStock

WB, ELISA Host: Rabbit Polyclonal unconjugated

Western blot analysis of V5 Tag antibody and 12 tagged protein lysate. (V5 Epitope Tag antibody)

Lanes: 1: non-transfected cells; 2: V5-tagged empty plasmid; and V5-tagged proteins: 3-A, 4-B, 5-C, 6-D; Data courtesy of Dr. (V5 Epitope Tag antibody)

Lanes: 1: non-transfected cells (V5 Epitope Tag antibody)

3 images

(1 reference)

Catalog No. ABIN3029565

Datasheet as PDF

Target See all V5 Epitope Tag products

V5 Epitope Tag

Reactivity

Please inquire
Host
62

44

7

5

4

1

1
Rabbit
Compatible Secondaries
Clonality
61

50

10
Polyclonal
Conjugate
57

7

7

6

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This V5 Epitope Tag antibody is un-conjugated
Application
90

47

37

35

23

20

13

12

7

6

6

6

6

2

2

2

1

1

1

1

1
Western Blotting (WB), ELISA

Sequence

GKPIPNPLLG LDST

Purification

Purified

Immunogen

The V5 sequence GKPIPNPLLGLDST was used as the immunogen for the V5 tag antibody.

Isotype

Ig Fraction
anti-V5 Epitope Tag antibody
WB, ELISA, IP, IF, FACS Host: Mouse Monoclonal 8A7A8 unconjugated

anti-V5 Epitope Tag antibody
WB Host: Goat Polyclonal unconjugated

anti-V5 Epitope Tag antibody
WB, IP, IF Host: Mouse Monoclonal 8G5 unconjugated

anti-V5 Epitope Tag antibody
WB, ELISA, IP Host: Rabbit Polyclonal unconjugated

anti-V5 Epitope Tag antibody
WB Host: Rabbit Polyclonal RB0413 unconjugated

anti-V5 Epitope Tag antibody
WB, ELISA, IP, IHC (fro), RIA, IHC Host: Mouse Monoclonal SV5-Pk1 unconjugated

anti-V5 Epitope Tag antibody
WB, IP, IF, IC Host: Mouse Monoclonal unconjugated

anti-V5 Epitope Tag antibody
WB, ELISA, IP, IF, IHC (fro), RIA, FACS Host: Mouse Monoclonal SV5-Pk1 unconjugated

anti-V5 Epitope Tag antibody
WB, ELISA, IP, DB Host: Mouse Monoclonal E10 unconjugated

anti-V5 Epitope Tag antibody
WB, ELISA, IHC Host: Mouse Monoclonal E10 unconjugated

Application Notes

Titration of the V5 Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions

For Research Use only
Format

Liquid

Buffer

In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Aliquot the V5 Tag antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Aagaard, Fischer, Lober, Neumann, Allahverdi, Sivelle, Miehe, Spillner: "Extract-Shaped Immune Repertoires as Source for Nanobody-Based Human IgE in Grass Pollen Allergy." in: Molecular biotechnology, Vol. 65, Issue 9, pp. 1518-1527, (2023) (PubMed).
Target

V5 Epitope Tag

Alternative Name

V5 Tag (V5 Epitope Tag Products)

Target Type

Tag

Background

Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). NSJBio's anti-V5 tag polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells.