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CD40 Ligand antibody (AA 33-62)

Name: CD40 Ligand antibody (AA 33-62)
SKU: ABIN3028551
Price: 606.92 USD
Availability: InStock

CD40LG Reactivity: Human WB, FACS, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

Western blot analysis of TRAP antibody and NCI-H460 lysate (CD40 Ligand antibody (AA 33-62))

TRAP antibody IHC analysis in formalin fixed and paraffin embedded human kidney tissue (CD40 Ligand antibody (AA 33-62))

IHC

Confocal immunofluorescent analysis of TRAP antibody with NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (CD40 Ligand antibody (AA 33-62))

TRAP antibody flow cytometric analysis of NCI-H460 cells (green) compared to a negative control (blue). (CD40 Ligand antibody (AA 33-62))

FACS

6 images

Catalog No. ABIN3028551

Datasheet as PDF

Target See all CD40 Ligand (CD40LG) Antibodies

CD40 Ligand (CD40LG)
Binding Specificity
16

10

8

7

6

4

4

4

4

4

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 33-62
Reactivity
152

81

34

9

8

6

5

2

2

2

2

1

1

1

1

1
Human
Host
96

66

22

15

12

1
Rabbit
Compatible Secondaries
Clonality
113

93

4
Polyclonal
Conjugate
105

23

21

14

8

3

3

3

3

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD40 Ligand antibody is un-conjugated
Application
109

100

52

50

39

29

20

17

14

14

13

13

5

4

4

4

3

3

2

2

2

1

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Cross-Reactivity (Details)

Expected species reactivity: Bovine, Pig

Purification

Purified

Immunogen

A portion of amino acids 33-62 from the human protein was used as the immunogen for this TRAP antibody.

Isotype

Ig Fraction

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anti-CD40 Ligand (CD40LG) (AA 1-261) antibody
CD40LG Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD40 Ligand (CD40LG) (AA 108-261) antibody
CD40LG Reactivity: Human FACS, IHC, ELISA, IF, Coat, StM Host: Mouse Monoclonal CD40LG-2763 unconjugated

anti-CD40 Ligand (CD40LG) (AA 108-261) antibody
CD40LG Reactivity: Human FACS, IHC, ELISA, IF, Coat, StM Host: Mouse Monoclonal CD40LG-2761 unconjugated

anti-CD40 Ligand (CD40LG) antibody (FITC)
CD40LG Reactivity: Mouse FACS Host: Armenian Hamster Monoclonal MR-1 FITC

anti-CD40 Ligand (CD40LG) (AA 33-62), (N-Term) antibody
CD40LG Reactivity: Human WB, FACS, IF, IHC (p) Host: Rabbit Polyclonal RB19150 unconjugated

anti-CD40 Ligand (CD40LG) (AA 79-129) antibody
CD40LG Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-CD40 Ligand (CD40LG) (AA 111-261) antibody
CD40LG Reactivity: Pig WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD40 Ligand (CD40LG) antibody (PE)
CD40LG Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal 24-31 PE

anti-CD40 Ligand (CD40LG) (AA 47-261) antibody
CD40LG Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 8H10F5 unconjugated

anti-CD40 Ligand (CD40LG) (AA 47-261) antibody
CD40LG Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 5A3A9 unconjugated

Application Notes

Titration of the TRAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions

For Research Use only
Format

Liquid

Buffer

In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Aliquot the TRAP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target

CD40 Ligand (CD40LG)

Alternative Name

TRAP (CD40L) (CD40LG Products)

Synonyms

CD154 antibody, cd40l antibody, CD40L antibody, HIGM1 antibody, IGM antibody, IMD3 antibody, T-BAM antibody, TNFSF5 antibody, TRAP antibody, gp39 antibody, hCD40L antibody, CD40-L antibody, Cd40l antibody, Ly-62 antibody, Ly62 antibody, Tnfsf5 antibody, CD40 ligand antibody, TNF superfamily member 5 antibody, CD40LG antibody, cd40l antibody, Cd40lg antibody

Background

TRAP is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in its gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.

UniProt

P29965

Pathways

NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints